Capillary isotachophoresis with electrospray-ionization mass-spectrometric detection: Cationic electrolyte systems in the medium- alkaline range for selective analysis of medium strong bases

0524028 - ÚIACH 2021 RIV NL eng J - Journal Article
Malá, Zdeňka - Gebauer, Petr
Capillary isotachophoresis with electrospray-ionization mass-spectrometric detection: Cationic electrolyte systems in the medium- alkaline range for selective analysis of medium strong bases.
Journal of Chromatography A. Roč. 1618, May (2020), č. článku 460907. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GA18-13135S
Institutional support: RVO:68081715
Keywords : capillary isotachophoresis * dried blood spots * ESI–MS detection * medium-alkaline pH
OECD category: Analytical chemistry
Impact factor: 4.759, year: 2020
Method of publishing: Limited access

This work extends the present working range of isotachophoresis (ITP) with electrospray-ionization mass-spectrometric (ESI–MS) detection and describes for the first time a functional cationic electrolyte system for analyses at medium-alkaline pH. So far no ITP–MS application was published on the analysis of medium strong bases although there is a broad spectrum of potential analytes like biogenic amines, alkaloids or drugs, where this technique promises interesting gains in both sensitivity and specificity. The presented results include a selection of suitable sufficiently volatile ESI-compatible system components, discussion of factors affecting system properties, and recommendations for functional ITP electrolyte systems. Theoretical conclusions based on calculations and computer simulations are confirmed by experiments with a model mixture of beta-blockers. Practical applicability of the method is demonstrated on the example of analysis of sotalol in dried blood spots where direct injection of aqueous extract, ITP stacking and MS detection provide a fast, simple and sensitive technique with limits of quantitation on the sub-nM level.
Permanent Link: http://hdl.handle.net/11104/0308342