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The repeated cytogenetic analysis of subjects occupationally exposed to nanoparticles: a pilot study
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SYSNO ASEP 0517729 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The repeated cytogenetic analysis of subjects occupationally exposed to nanoparticles: a pilot study Author(s) Rössnerová, Andrea (UEM-P) RID
Pelclová, D. (CZ)
Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
Rössner ml., Pavel (UEM-P) RID, ORCID
Elzeinova, Fatima (UEM-P)
Vrbová, Kristýna (UEM-P)
Topinka, Jan (UEM-P) RID, ORCID
Schwarz, Jaroslav (UCHP-M) RID, ORCID, SAI
Ondráček, Jakub (UCHP-M) RID, ORCID, SAI
Koštejn, Martin (UCHP-M) RID, SAI, ORCID
Komarc, M. (CZ)
Vlčková, Š. (CZ)
Fenclová, Z. (CZ)
Dvořáčková, Š. (CZ)Source Title Mutagenesis. - : Oxford University Press - ISSN 0267-8357
Roč. 34, č. 3 (2019), s. 253-263Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords peripheral-blood lymphocytes ; oxidative stress biomarkers ; exhaled breath condensate Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) Subject RIV - cooperation Institute of Chemical Process Fundamentals - Air Pollution ; Quality R&D Projects GA18-02079S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access with time embargo (25.06.2020) Institutional support UEM-P - RVO:68378041 ; UCHP-M - RVO:67985858 UT WOS 000488104300004 EID SCOPUS 85072508853 DOI 10.1093/mutage/gez016 Annotation The application of nanomaterials has been rapidly increasing during recent years. Inhalation exposure to nanoparticles (NP) may result in negative toxic effects but there is a critical lack of human studies, especially those related to possible DNA alterations. We analyzed pre-shift and post-shift a group of nanocomposite researchers with a long-term working background (17.8 +/- 10.0 years) and matched controls. The study group consisted of 73.2% males and 26.8% females. Aerosol exposure monitoring during a working shift (involving welding, smelting, machining) to assess the differences in exposure to particulate matter (PM) including nanosized fractions <25-100 nm, and their chemical analysis, was carried out. A micronucleus assay using Human Pan Centromeric probes, was applied to distinguish between the frequency of centromere positive (CEN+) and centromere negative (CEN-) micronuclei (MN) in the binucleated cells. This approach allowed recognition of the types of chromosomal damage: losses and breaks. The monitoring data revealed differences in the exposure to NP related to individual working processes, and in the chemical composition of nanofraction. The cytogenetic results of this pilot study demonstrated a lack of effect of long-term (years) exposure to NP (total frequency of MN, P = 0.743), although this exposure may be responsible for DNA damage pattern changes (12% increase of chromosomal breaks-clastogenic effect). Moreover, short-term (daily shift) exposure could be a reason for the increase of chromosomal breaks in a subgroup of researchers involved in welding and smelting processes (clastogenic effect, P = 0.037). The gender and/or gender ratio of the study participants was also an important factor for the interpretation of the results. As this type of human study is unique, further research is needed to understand the effects of long-term and short-term exposure to NP. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2020 Electronic address https://academic.oup.com/mutage/article-abstract/34/3/253/5522409?redirectedFrom=fulltext
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