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Ganoderma Lucidum induces oxidative DNA damage and enhances the effect of 5-Fluorouracil in colorectal cancer in vitro and in vivo
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SYSNO ASEP 0517497 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ganoderma Lucidum induces oxidative DNA damage and enhances the effect of 5-Fluorouracil in colorectal cancer in vitro and in vivo Author(s) Opattová, Alena (UEM-P)
Horák, Josef (UEM-P) ORCID
Vodenková, Soňa (UEM-P) ORCID, RID
Kostovčíková, Klára (MBU-M) ORCID
Čumová, Andrea (UEM-P)
Macinga, P. (CZ)
Galanová, Natálie (MBU-M) ORCID
Rejhová, Alexandra (UEM-P)
Vodičková, Ludmila (UEM-P) RID
Kozicz, K. (SK)
Turnovcová, Karolína (UEM-P) ORCID
Hucl, T. (CZ)
Slíva, Daniel (UEM-P)
Vodička, Pavel (UEM-P) RIDArticle number UNSP 403065 Source Title Mutation Research - Genetic Toxicology and Environmental Mutagenesis. - : Elsevier - ISSN 1383-5718
Roč. 845, SI (2019)Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords ganoderma lucidum ; colorectal cancer ; DNA damage Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology Subject RIV - cooperation Institute of Microbiology - Immunology R&D Projects GA18-09709S GA ČR - Czech Science Foundation (CSF) GA19-10543S GA ČR - Czech Science Foundation (CSF) NV15-27580A GA MZd - Ministry of Health (MZ) Method of publishing Open access Institutional support UEM-P - RVO:68378041 ; MBU-M - RVO:61388971 UT WOS 000489193400017 EID SCOPUS 85066812771 DOI 10.1016/j.mrgentox.2019.06.001 Annotation The first-line chemotherapy of colorectal cancer (CRC), besides surgery, comprises administration of 5-Fluorouracil (5FU). Apart from cytotoxic effect on cancer cells, 5FU may also cause adverse side effects. Ganoderma Lucidum (GLC) is a mushroom used in Traditional Eastern Medicine. We propose that natural compounds, particularly GLC extracts, may sensitize cancer cells to conventional chemotherapeutics. This combination therapy could lead to more selective cancer cell death and may improve the response to the therapy and diminish the adverse effects of anticancer drugs.
Here we demonstrate that GLC induced oxidative DNA damage selectively in colorectal cancer cell lines, whereas it protected non-malignant cells from the accumulation of reactive oxygen species. Accumulation of DNA damage caused sensitization of cancer cells to 5FU resulting in improved anticancer effect of 5FU. The results obtained in colorectal cell lines were confirmed in in vivo study: GLC co-treatment with 5FU increased the survival of treated mice and reduced the tumor volume in comparison with group treated with 5FU alone.
Combination of conventional chemotherapeutics and natural compounds is a promising approach, which may reduce the effective curative dose of anticancer drugs, suppress their adverse effects and ultimately lead to better quality of life of CRC patients.Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S1383571819300944?via%3Dihub
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