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Measurement of protein and sugar consumed by bumblebee larvae under standard and food stress conditions using lanthanide complexes
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SYSNO ASEP 0505473 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Measurement of protein and sugar consumed by bumblebee larvae under standard and food stress conditions using lanthanide complexes Author(s) Macháčková, L. (CZ)
Votavová, A. (CZ)
Mikát, M. (CZ)
Matějková, Stanislava (UOCHB-X) RID, ORCID
Řehoř, Ivan (UOCHB-X) RID, ORCID
Gillarová, S. (CZ)
Straka, J. (CZ)Source Title Insectes Sociaux. - : Springer - ISSN 0020-1812
Roč. 66, č. 2 (2019), s. 245-256Number of pages 12 s. Language eng - English Country CH - Switzerland Keywords sugar ; protein ; food stress ; marking technique ; lanthanides ; Apidae Subject RIV EG - Zoology OECD category Entomology Method of publishing Limited access Institutional support UOCHB-X - RVO:61388963 UT WOS 000466965700008 EID SCOPUS 85057957187 DOI 10.1007/s00040-018-00681-w Annotation Measurement of food consumed by larvae of progressive provisioning bees requires an experimental approach that marks the food sources appropriately. This paper aims to measure the amount of sugars and proteins consumed by a single bumblebee larva and to define, how the proportion between protein and sugar changes under stress conditions. We marked sugar and pollen sources using lanthanide (gadolinium, dysprosium) complexes with diethylene-triamine-pentaacetic acid (DTPA) chelator. We precisely quantified the amount of Gd and Dy in feces, and thus quantified linearly correlated protein and sugar consumed by males and females (workers) of Bombus terrestris during their development. We compared body mass, total amount of ingested sugar and total amount of ingested protein in colonies which are fed ad libitum and in colonies with restricted feeding. Males fed ad libitum during their development ingested 0.52mg of protein and 4.43mg of sugar, and workers fed ad libitum ingested 0.54mg of protein and 4.26mg of sugar per 1 mg of dry body weight on average. Food-stressed workers ingested 0.51mg of protein and 4.65mg of sugar per 1 mg of dry body weight on average. Strong positive correlation between body mass, total protein ingested, and total sugar ingested in all our experiments was present. However, the consumption of sugar grew steeper with the consumption of protein in colonies under food stress compared to the ad libitum-fed colonies. In conclusion, we show the existence of different patterns in ingestion of protein and sugar between well-fed and food-stressed bumblebee colonies. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2020 Electronic address https://link.springer.com/article/10.1007%2Fs00040-018-00681-w
Number of the records: 1