Tubes for detection of cholinesterase inhibitors - unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers

Zeman, J.; Vetchý, D.; Pavloková, S.; Franc, A.; Pitschmann, V.; Dominik, M.; Urbanová, Martina; Šeděnková, Ivana

doi:https://dx.doi.org/10.1016/j.enzmictec.2019.05.002

Number of the records: 1

Tubes for detection of cholinesterase inhibitors - unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers

1.

SYSNO ASEP	0504873
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Tubes for detection of cholinesterase inhibitors - unique effects of Neusilin on the stability of butyrylcholinesterase-impregnated carriers
Author(s)	Zeman, J. (CZ) Vetchý, D. (CZ) Pavloková, S. (CZ) Franc, A. (CZ) Pitschmann, V. (CZ) Dominik, M. (CZ) Urbanová, Martina (UMCH-V)_{RID, ORCID} Šeděnková, Ivana (UMCH-V)_RID
Source Title	Enzyme and Microbial Technology. - : Elsevier - ISSN 0141-0229 Roč. 128, September (2019), s. 26-33
Number of pages	8 s.
Language	eng - English
Country	US - United States
Keywords	cholinesterase ; detection ; enzyme
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
Method of publishing	Limited access
Institutional support	UMCH-V - RVO:61389013
UT WOS	000472988700004
EID SCOPUS	85065427273
DOI	10.1016/j.enzmictec.2019.05.002
Annotation	Detection tubes are small devices for the colorimetric enzymatic detection of cholinesterase inhibitors such as sarin, soman, VX nerve agents and substances denoted as Novichok. These detectors contain carriers in the form of pellets with immobilized cholinesterase, substrate and detection reagent. Their advantages are portability, sensitivity and simplicity, enabling fast detection of such compounds from air and water in case of a terrorist attack or war. In general, maintaining the stability of an enzyme for a longer time is very problematic. Therefore, its further enhancement is required for safety and financial reasons. In this study, the stability of our patented carriers in the form of pellets with immobilized butyrylcholinesterase containing an increasing amount of the unique sorbent Neusilin® US2 was evaluated. The samples containing Neusilin maintained the stability of the immobilized enzyme for a longer time even at higher temperature and humidity than the currently commercially used carrier without Neusilin, allowing improved detection of nerve agents.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2020
Electronic address	https://www.sciencedirect.com/science/article/pii/S0141022919300699?via%3Dihub

Number of the records: 1