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Substitution-Inert Polynuclear Platinum Complexes Act as Potent Inducers of Condensation/Aggregation of Short Single- and Double-Stranded DNA and RNA Oligonucleotides
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SYSNO ASEP 0504519 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Substitution-Inert Polynuclear Platinum Complexes Act as Potent Inducers of Condensation/Aggregation of Short Single- and Double-Stranded DNA and RNA Oligonucleotides Author(s) Malina, Jaroslav (BFU-R) ORCID
Farrell, N.P. (US)
Brabec, Viktor (BFU-R) RID, ORCIDNumber of authors 3 Source Title Chemistry - A European Journal. - : Wiley - ISSN 0947-6539
Roč. 25, č. 12 (2019), s. 2995-2999Number of pages 5 s. Publication form Print - P Language eng - English Country DE - Germany Keywords phosphate clamp ; recognition ; selectivity ; length Subject RIV CE - Biochemistry OECD category Physical chemistry R&D Projects GA16-03517S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000460678500013 DOI 10.1002/chem.201806276 Annotation Compounds condensing DNA and RNA molecules can essentially affect important biological processes including DNA replication and transcription. Here, this work shows with the aid of total intensity light scattering, gel electrophoresis, and atomic force microscopy (AFM) that the substitution-inert polynuclear platinum complexes (SI-PPCs), particularly [{trans-Pt(NH3)(2)(NH2(CH2)(6)- NH3+)}(2)--{trans-Pt(NH3)(2)(NH2(CH2)(6)NH2)(2)}](8+) (Triplatin NC), exhibit an unprecedented high potency to condense/aggregate fragments of DNA and RNA as short as 20 base pairs. SI-PPCs condensates are distinctive from those generated by the naturally occurring polyamines (commonly used DNA compacting/condensing agents). Collectively, the results further confirm that SI-PPCs are very efficient inducers of condensation of DNA and RNA, including their short fragments that might have potential in gene therapy, biotechnology, and bionanotechnology. Moreover, the data confirm the structural advantages of the phosphate clamp, with a well-defined rigid DNA recognition motif in initiating condensation and aggregation phenomena on oligonucleotides. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020 Electronic address https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.201806276
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