Zwitterionic functionalizable scaffolds with gyroid pore architecture for tissue engineering

0504008 - ÚMCH 2020 RIV DE eng J - Journal Article
Kostina, N. Yu. - Blanquer, S. - Pop-Georgievski, Ognen - Rahimi, K. - Dittrich, B. - Höcherl, Anita - Michálek, Jiří - Grijpma, D. W. - Rodriguez-Emmenegger, C.
Zwitterionic functionalizable scaffolds with gyroid pore architecture for tissue engineering.
Macromolecular Bioscience. Roč. 19, č. 4 (2019), s. 1-9, č. článku 1800403. ISSN 1616-5187. E-ISSN 1616-5195
R&D Projects: GA ČR(CZ) GA16-02702S; GA ČR(CZ) GA16-04863S; GA MŠMT(CZ) LO1507
Institutional support: RVO:61389013
Keywords : zwitterionic scaffolds * tissue engineering * stereolithography
OECD category: Polymer science
Impact factor: 3.416, year: 2019
Method of publishing: Limited access
https://onlinelibrary.wiley.com/doi/full/10.1002/mabi.201800403

Stereolithography‐assisted fabrication of hydrogels of carboxybetaine methacrylamide (CBMAA) and a α,ω‐methacrylate poly(d,l‐lactide‐block‐ethylene glycol‐block‐ d,l‐lactide) (MA‐PDLLA‐PEG‐PDLLA‐MA) telechelic triblock macromer is presented. This technique allows printing complex structures with gyroid interconnected porosity possessing extremely high specific area. Hydrogels are characterized by infrared spectroscopy (FTIR), X‐ray photoelectron spectroscopy (XPS), and laser scanning confocal microscopy (LSCM). The copolymerization with zwitterionic comonomer leads hydrogels with high equilibrium water content (EWC), up to 700% while maintaining mechanical robustness. The introduction of carboxybetaine yields excellent resistance to nonspecific protein adsorption while providing a facile way for specific biofunctionalization with a model protein, fluorescein isothiocyanate labeled bovine serum albumin (BSA). The homogeneous protein immobilization across the hydrogel pores prove the accessibility to the innermost pore volumes. The remarkably low protein adsorption combined with the interconnected nature of the porosity allowing fast diffusion of nutrient and waste product and the mimicry of bone trabecular, makes the hydrogels presented here highly attractive for tissue engineering.
Permanent Link: http://hdl.handle.net/11104/0296303