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Advances in Chemical Biology
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SYSNO ASEP 0502873 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title The role of forward genetic screens in the identification of FAM208A as an epigenetic silencing factor Author(s) Epp, Trevor (UMG-J) RID
Schuster, Bjorn (UMG-J)
Chawengsaksophak, Kallayanee (UMG-J) ORCIDSource Title Advances in Chemical Biology. - Praha : OPTIO CZ, 2019 / Bartůněk Petr - ISBN 978-80-88011-03-3 Pages s. 50-59 Number of pages 9 s. Number of copy 50 Number of pages 210 Publication form Print - P Language eng - English Country CZ - Czech Republic Keywords FAM208A ; gene scilencing ; H3K9-methyltransferase Subject RIV EB - Genetics ; Molecular Biology OECD category Cell biology R&D Projects LO1220 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 Annotation A previously unknown gene, FAM208A, has now featured predominantly in several different genome-wide screens for modifiers of gene silencing. It is now known that FAM208A exists within a core epigenetic silencing complex comprising also MPHOSPH8 and PPHLN1. This complex, termed HUSH (human silencing hub), binds pre-existing, repressive H3K9me3 marks and then catalyses the local spread of these same marks through the recruitment of the H3K9-methyltransferase, SETDB1. Here we discuss the role of forward genetic screens in the discovery of FAM208A functions and the prospects of employing forward chemical genetics screens to further probe its mechanism of action. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2019
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