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Electrophoretic focusing on inverse electromigration dispersion gradient. The fundamental resolution equation and pressure-assisted performance enhancement
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SYSNO ASEP 0490545 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Electrophoretic focusing on inverse electromigration dispersion gradient. The fundamental resolution equation and pressure-assisted performance enhancement Author(s) Malá, Zdeňka (UIACH-O) RID
Gebauer, Petr (UIACH-O) RID, ORCIDNumber of authors 2 Source Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1563, AUG (2018), s. 191-197Number of pages 7 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords capillary electrophoresis ; focusing ; sulfonamides ; chlorophenols Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA16-09135S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 UT WOS 000437814700019 EID SCOPUS 85048483721 DOI 10.1016/j.chroma.2018.05.076 Annotation Electrophoretic focusing on inverse electromigration dispersion (EMD) gradient is a new analytical technique based on a unique separation principle where weak non-amphoteric ionogenic species are focused, separated and transported to the detector by an EMD profile of suitable properties. The present work extends the theoretical description of this method by introducing the concept of resolution and deriving the fundamental equation expressing resolution as function of basic system parameters. The results indicate that at constant current operation, resolution is proportional to the square root of time. For variable current regimes (e.g. constant voltage), the time variable is replaced by the product of electric current and passed electric charge. Computer simulations for a model pair of substances support the validity of the presented theory and confirm the theoretical conclusion that resolution can be increased by allowing longer electromigration of the gradient in terms of time or passed charge. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2019
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