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Peripheral inflammation affects modulation of nociceptive synaptic transmission in the spinal cord induced by N‐arachidonoylphosphatidylethanolamine
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SYSNO ASEP 0489989 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Peripheral inflammation affects modulation of nociceptive synaptic transmission in the spinal cord induced by N‐arachidonoylphosphatidylethanolamine Author(s) Nerandžič, Vladimír (FGU-C)
Mrózková, Petra (FGU-C) RID, ORCID
Adámek, Pavel (FGU-C) RID, ORCID, SAI
Špicarová, Diana (FGU-C) RID, ORCID
Nagy, I. (GB)
Paleček, Jiří (FGU-C) RID, ORCIDSource Title British Journal of Pharmacology. - : Wiley - ISSN 0007-1188
Roč. 175, č. 12 (2018), s. 2322-2356Number of pages 15 s. Language eng - English Country GB - United Kingdom Keywords Anandamide ; TRPV1 ; pain ; cannabinoids Subject RIV FH - Neurology OECD category Neurosciences (including psychophysiology R&D Projects GA15-11138S GA ČR - Czech Science Foundation (CSF) LH15279 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FGU-C - RVO:67985823 UT WOS 000434071600015 EID SCOPUS 85020379780 DOI 10.1111/bph.13849 Annotation Endocannabinoids play an important role in modulating spinal nociceptive signalling, crucial for the development of pain. The cannabinoid CB1 receptor and the TRPV1 cation channel are both activated by the endocannabinoid anandamide, a product of biosynthesis from the endogenous lipid precursor N‐arachidonoylphosphatidylethanolamine (20:4‐NAPE). Here, we report CB1 receptor‐ and TRPV1‐mediated effects of 20:4‐NAPE on spinal synaptic transmission in control and inflammatory conditions. Spontaneous (sEPSCs) and dorsal root stimulation‐evoked (eEPSCs) excitatory postsynaptic currents from superficial dorsal horn neurons in rat spinal cord slices were assessed. Peripheral inflammation was induced by carrageenan. Anandamide concentration was assessed by mass spectrometry. While 20:4‐NAPE treatment inhibited the excitatory synaptic transmission in both naive and inflammatory conditions, peripheral inflammation altered the underlying mechanisms. Our data indicate that 20:4‐NAPE application induced mainly CB1 receptor‐mediated inhibitory effects in naive animals while TRPV1‐mediated mechanisms were also involved after inflammation. Increasing anandamide levels for analgesic purposes by applying substrate for its local synthesis may be more effective than systemic anandamide application or inhibition of its degradation. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2019
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