Using Odd-Alkanes as a Carbon Source to Increase the Content of Nutritionally Important Fatty Acids in Candida krusei, Trichosporon cutaneum, and Yarrowia lipolytica

0481391 - MBÚ 2018 RIV US eng J - Journal Article
Matátková, O. - Gharwalová, L. - Zimola, M. - Řezanka, Tomáš - Masák, J. - Kolouchová, I.
Using Odd-Alkanes as a Carbon Source to Increase the Content of Nutritionally Important Fatty Acids in Candida krusei, Trichosporon cutaneum, and Yarrowia lipolytica.
International Journal of Analytical Chemistry. Roč. 2017, October (2017), s. 1-9, č. článku 8195329. ISSN 1687-8760. E-ISSN 1687-8779
R&D Projects: GA ČR(CZ) GA17-00027S
Institutional support: RVO:61388971
Keywords : HYDROCARBON-UTILIZING MICROORGANISMS * CHAIN N-ALKANES * POTENTIAL APPLICATIONS
OECD category: Microbiology
Impact factor: 1.479, year: 2017

We investigated the possibility of utilizing unusual carbon sources by three yeast strains: Candida krusei DBM 2136, Trichosporon cutaneum CCY 30-5-10, and Yarrowia lipolytica CCY 30-26-36. These strains are characterized by high biomass yield, ability to accumulate high amounts of lipids, and their potential as producers of dietetically important fatty acids. The aim of this work was the production of nutritionally important fatty acids by utilization of n-alkanes with an odd number of carbon atoms, alone and in combination with glucose and subsequent analysis of microbial lipids accumulation and fatty acid profile. All three yeast strains were able to grow and produce high amounts of the fatty acids of interest. Yarrowia lipolytica was found as themost suitable strain for the growth on n-alkanes (n-pentadecane and n-heptadecane) as the only source of carbon. The addition of biosurfactants rhamnolipids into the cultivation increased the ratio of heptadecenoic acid (up to 17.9% of total FAs in Y. lipolytica CCY 30-2636, 14.9% in T. cutaneum CCY 30-5-10, and 17.5% in C. krusei DBM 2136) and the total biomass yield. The results show that, by manipulation of the initial cultivation conditions, the ratio of important fatty acids may be increased.
Permanent Link: http://hdl.handle.net/11104/0277027