Immunoassays of chemically modified polysaccharides, glycans in glycoproteins and ribose in nucleic acids

Strmečki, S.; Trefulka, Mojmír; Zatloukalová, P.; Durech, M.; Vojtěšek, B.; Paleček, Emil

doi:https://dx.doi.org/10.1016/j.aca.2016.12.013

Number of the records: 1

Immunoassays of chemically modified polysaccharides, glycans in glycoproteins and ribose in nucleic acids

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SYSNO ASEP	0476719
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Immunoassays of chemically modified polysaccharides, glycans in glycoproteins and ribose in nucleic acids
Author(s)	Strmečki, S. (HR) Trefulka, Mojmír (BFU-R)_{RID, ORCID} Zatloukalová, P. (CZ) Durech, M. (CZ) Vojtěšek, B. (CZ) Paleček, Emil (BFU-R)_{RID, ORCID}
Number of authors	6
Source Title	Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670 Roč. 955, FEB2017 (2017), s. 108-115
Number of pages	8 s.
Publication form	Print - P
Language	eng - English
Country	NL - Netherlands
Keywords	structural-characterization ; voltammetric determination ; electrochemical detection
Subject RIV	CB - Analytical Chemistry, Separation
OECD category	Analytical chemistry
R&D Projects	GA15-15479S GA ČR - Czech Science Foundation (CSF)
Institutional support	BFU-R - RVO:68081707
UT WOS	000393242700010
DOI	10.1016/j.aca.2016.12.013
Annotation	Glycosylation of proteins plays an important role in health and diseases. At present new simple and inexpensive methods of glycoprotein analysis are sought. We developed a monoclonal antibody Manost 2.1 in mice after immunization with the adduct of mannan with Os(VI)temed complex (temed is N,N,N',N'-tetramethylethylenediamine). The specificity of this antibody to different biomolecules treated with Os(VI)temed was tested using dot blot immunoassay. Manost 2.1 showed specificity toward Os(VI) temed-modified polysaccharides, glycoproteins and ribonucleotide at the 3'-end in DNA. The antibody recognized neither the unmodified compounds nor the non-glycosylated proteins treated with Os(VI) temed. We also performed western blotting and Coomassie silver blue staining of mixtures of biomacromolecules treated with Os(VI)temed and identified specifically the modified glycoproteins. The immunochemical method using Manost 2.1 was compared with electrochemical analyses based on redox signals of the Os(VI)temed adducts, with similar results in terms of sensitivity. This new antibody-based approach opens the door for rapid and inexpensive analysis of glycans and glycoproteins in various scientific and medical fields, including cancer research and the future application of glycoprotein detection in clinical practice. (C) 2016 Elsevier B.V. All rights reserved.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2018

Number of the records: 1