Number of the records: 1
Development of intron targeting (IT) markers specific for chromosome arm 4VS of Haynaldia villosa by chromosome sorting and next-generation sequencing
- 1.
SYSNO ASEP 0476326 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Development of intron targeting (IT) markers specific for chromosome arm 4VS of Haynaldia villosa by chromosome sorting and next-generation sequencing Author(s) Wang, H. (CN)
Dai, K. (CN)
Xiao, J. (CN)
Yuan, C. (CN)
Zhao, R. L. (CN)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Wu, Y. (CN)
Cao, A. (CN)
Chen, P. (CN)
Zhang, S. (CN)
Wang, X. (CN)Number of authors 11 Article number 167 Source Title BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 18, FEB 15 (2017)Number of pages 9 s. Language eng - English Country US - United States Keywords triticum-aestivum l. ; conferring resistance ; wild relatives ; mosaic-virus ; plug markers ; bread wheat ; genome ; gene ; rye ; improvement ; Triticum aestivum ; Haynaldia villosa ; Molecular marker ; Intron polymorphism Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000394385700003 DOI 10.1186/s12864-017-3567-z Annotation Background: Haynaldia villosa (L.) Schur (syn. Dasypyrum villosum L. Candargy, 2n = 14, genome VV) is the tertiary gene pool of wheat, and thus a potential resource of genes for wheat improvement. Among other, wheat yellow mosaic (WYM) resistance gene Wss1 and a take-all resistance gene were identified on the short arm of chromosome 4 V (4VS) of H. villosa. We had obtained introgressions on 4VS chromosome arm, with the objective of utilizing the target genes. However, monitoring these introgressions has been a daunting task because of inadequate knowledge as to H. villosa genome, as reflected by the lack of specific markers.
Results: This study aims to develop 4VS-specific markers by combination of chromosome sorting and next-generation sequencing. The short arm of chromosome 4VS of H. villosa was flow-sorted using a FACSVantage SE flow cytometer and sorter, and then sequenced by Illumina sequencing. The sequence of H. villosa 4VS was assembled by the software Hecate, and then was compared with the sequence assemblies of wheat chromosome arms 4AL, 4BS and 4DS and Ae. tauschii 4DS, with the objectives of identifying exon-exon junctions and localizing introns on chromosome 4VS of H. villosa. The intron length polymorphisms suitable for designing H. villosa primers were evaluated with criteria. Consequently, we designed a total of 359 intron targeting (IT) markers, among which 232 (64.62%) markers were specific for tracing the 4VS chromatin in the wheat background.
Conclusion: The combination of chromosome sorting and next-generation sequencing to develop specific IT markers for 4VS of H. villosa has high success rate and specificity, thus being applicable for the development of chromosome-specific markers for alien chromatin in wheat breeding.Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
Number of the records: 1