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Influence of commensal Lactobacillus amylovorus, probiotic E.coli Nissle 1917 and Salmonella Typhimurium on transcriptions of claudin-1 and occludin in the intestine of gnotobiotic piglets

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    0469676 - MBÚ 2017 TH eng A - Abstract
    Šplíchalová, Alla - Šplíchal, Igor - Slavíková, Věra
    Influence of commensal Lactobacillus amylovorus, probiotic E.coli Nissle 1917 and Salmonella Typhimurium on transcriptions of claudin-1 and occludin in the intestine of gnotobiotic piglets.
    Microbes for the Benefits of Mankind, Book of abstracts. Phuket: ICOBM, 2016, 2016. s. 139-139. ISBN 1876-2883.
    [International Conference on Beneficial Microbes 2016. 31.05.2016-16.06.2016, Phuket]
    R&D Projects: GA MŠMT(CZ) LD15090
    Institutional support: RVO:61388971
    Keywords : Lactobacillus amylovorus * c E.coli Nissle 1917 * Salmonella Typhimurium
    Subject RIV: EC - Immunology

    Colostrum-deprived germ-free piglets (GF) were colonized with commensal Lactobacillus amylovorus (LA) or probiotic E. coli Nissle 1917 (EcN) four hours after hysterectomy. Another group of piglets was infected at one week of age with Salmonella Typhimurium (ST) for 24 hours. Bacterial colonization of the intestine of one-week-old gnotobiotic piglets and possible translocation (CFU counting), transcriptions (RT-qPCR) of claudin-1 and occludin, and their protein expression (IF) were evaluated. Surprisingly the transcriptions of claudin-1 and occludin in Salmonella-infected piglets showed quite opposite trend compare to GF piglets
    Permanent Link: http://hdl.handle.net/11104/0268286

     
     
Number of the records: 1  

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