Autocrine effects of transgenic resistin reduce palmitate and glucose oxidation in brown adipose tissue

Pravenec, Michal; Mlejnek, Petr; Zídek, Václav; Landa, Vladimír; Šimáková, Miroslava; Šilhavý, Jan; Strnad, Hynek; Eigner, Sebastian; Eigner-Henke, Kateřina; Škop, V.; Malínská, H.; Trnovská, J.; Kazdová, L.; Drahota, Zdeněk; Mráček, Tomáš; Houštěk, Josef

doi:https://dx.doi.org/10.1152/physiolgenomics.00122.2015

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Autocrine effects of transgenic resistin reduce palmitate and glucose oxidation in brown adipose tissue

1.

SYSNO ASEP	0460150
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Autocrine effects of transgenic resistin reduce palmitate and glucose oxidation in brown adipose tissue
Author(s)	Pravenec, Michal (FGU-C)_{RID, ORCID} Mlejnek, Petr (FGU-C)_{RID, ORCID} Zídek, Václav (FGU-C)_RID Landa, Vladimír (FGU-C)_RID Šimáková, Miroslava (FGU-C)_{RID, ORCID} Šilhavý, Jan (FGU-C)_{RID, ORCID} Strnad, Hynek (UMG-J)_RID Eigner, Sebastian (UJF-V)_RID Eigner-Henke, Kateřina (FGU-C) Škop, V. (CZ) Malínská, H. (CZ) Trnovská, J. (CZ) Kazdová, L. (CZ) Drahota, Zdeněk (FGU-C)_{RID, ORCID} Mráček, Tomáš (FGU-C)_{RID, ORCID} Houštěk, Josef (FGU-C)_{RID, ORCID}
Source Title	Physiological Genomics. - : American Physiological Society - ISSN 1094-8341 Roč. 48, č. 6 (2016), s. 420-427
Number of pages	8 s.
Language	eng - English
Country	US - United States
Keywords	brown adipose tissue ; autocrine ; transgenic ; spontaneously hypertensive rat
Subject RIV	FB - Endocrinology, Diabetology, Metabolism, Nutrition
R&D Projects	LL1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GB14-36804G GA ČR - Czech Science Foundation (CSF)
	NT14325 GA MZd - Ministry of Health (MZ)
Institutional support	FGU-C - RVO:67985823 ; UMG-J - RVO:68378050 ; UJF-V - RVO:61389005
UT WOS	000378338300005
EID SCOPUS	84983770971
DOI	10.1152/physiolgenomics.00122.2015
Annotation	Resistin has been originally identified as an adipokine that links obesity to insulin resistance in mice. In our previous studies in spontaneously hypertensive rats (SHR) expressing a nonsecreted form of mouse resistin (Retn) transgene specifically in adipose tissue (SHR-Retn), we have observed an increased lipolysis and serum free fatty acids, ectopic fat accumulation in muscles and insulin resistance. Recently, brown adipose tissue (BAT) has been suggested to play an important role in the pathogenesis of metabolic disturbances. In the current study, we have analyzed autocrine effects of transgenic resistin on BAT glucose and lipid metabolism and mitochondrial function in the SHR-Retn versus nontransgenic SHR controls. We observed that interscapular BAT isolated from SHR-Retn transgenic rats when compared to SHR controls showed a lower relative weight, significantly reduced both basal and insulin stimulated incorporation of palmitate into BAT lipids, and significantly decreased palmitate oxidation and glucose oxidation. Gene expression profiles in BAT identified differentially expressed genes involved in skeletal muscle and connective tissue development, inflammation and MAPK and insulin signaling. These results provide evidence that autocrine effects of resistin attenuate differentiation and activity of BAT and thus may play a role in the pathogenesis of insulin resistance in the rat.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2017

Number of the records: 1