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Phylogeny and morphological variability of trypanosomes from African pelomedusid turtles with redescription of Trypanosoma mocambicum Pienaar, 1962
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SYSNO ASEP 0454867 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Phylogeny and morphological variability of trypanosomes from African pelomedusid turtles with redescription of Trypanosoma mocambicum Pienaar, 1962 Author(s) Dvořáková, N. (CZ)
Čepička, I. (CZ)
Qablan, M. A. (CZ)
Gibson, W. (GB)
Blažek, Radim (UBO-W) RID, ORCID, SAI
Široký, P. (CZ)Number of authors 6 Source Title Protist. - : Elsevier - ISSN 1434-4610
Roč. 166, č. 6 (2015), s. 599-608Number of pages 10 s. Language eng - English Country DE - Germany Keywords Trypanosoma ; turtle ; Pelusios ; polymorphism ; phylogeny ; SSU rRNA gene Subject RIV EG - Zoology Institutional support UBO-W - RVO:68081766 UT WOS 000366806600001 EID SCOPUS 84947436474 DOI 10.1016/j.protis.2015.10.002 Annotation Little is known about host specificity, genetic diversity and phylogenetic relationships of African turtle trypanosomes. Using PCR targeting the SSU rRNA gene, we detected trypanosomes in 24 of 134 (17.9%) wild caught African pelomedusid turtles: Pelusios upembae (n = 14), P. bechuanicus (n = 1), P. rhodesianus (n = 3) and P. subniger (n = 6). Mixed infection of Trypanosoma species was confirmed by PCR in three specimens of P. upembae, and in one specimen each of P. bechuanicus, P. rhodesianus, and P. subniger. Microscopic examination of stained blood smears revealed two distinct forms (broad and slender) of trypomastigotes. The broad form coincided in morphology with T. mocambicum Pienaar, 1962. Accordingly, we have designated this form as the neotype of T. mocambicum. In phylogenetic analysis of the SSU rRNA gene, all the new turtle trypanosome sequences grouped in a single clade within the strongly supported "aquatic" clade of Trypanosoma species. The turtle trypanosome clade was further subdivided into two subclades, which did not correlate with host turtle species or trypanosome morphology. This study provides the first sequence data of Trypanosoma species isolated from freshwater turtles from tropical Africa and extends knowledge on diversity of trypanosomes in the Afrotropical zoogeographical realm. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2016
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