Number of the records: 1
Non-fouling biodegradable poly(.epsilon.-caprolactone) nanofibers for tissue engineering
- 1.
SYSNO ASEP 0454747 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Non-fouling biodegradable poly(.epsilon.-caprolactone) nanofibers for tissue engineering Author(s) Kostina, Nina Yu. (UMCH-V)
Pop-Georgievski, Ognen (UMCH-V) RID, ORCID
Bachmann, M. (DE)
Neykova, Neda (FZU-D) RID, ORCID
Bruns, M. (DE)
Michálek, Jiří (UMCH-V) RID, ORCID
Bastmeyer, M. (DE)
Rodriguez-Emmenegger, Cesar (UMCH-V) RIDSource Title Macromolecular Bioscience. - : Wiley - ISSN 1616-5187
Roč. 16, č. 1 (2016), s. 83-94Number of pages 12 s. Language eng - English Country DE - Germany Keywords antifouling ; cell adhesion ; nanofibers Subject RIV CD - Macromolecular Chemistry Subject RIV - cooperation Institute of Physics - Solid Matter Physics ; Magnetism R&D Projects GJ15-09368Y GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LH13178 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMCH-V - RVO:61389013 ; FZU-D - RVO:68378271 UT WOS 000368642300008 EID SCOPUS 84954290374 DOI 10.1002/mabi.201500252 Annotation Poly(ɛ-caprolactone) (PCL) nanofibers are very attractive materials for tissue engineering (TE) due to their degradability and structural similarity to the extracellular matrix (ECM). However, upon exposure to biological media, their surface is rapidly fouled by proteins and cells, which may lead to inflammation and foreign body reaction. In this study, an approach for the modification of PCL nanofibers to prevent protein fouling from biological fluids and subsequent cell adhesion is introduced. A biomimetic polydopamine (PDA) layer was deposited on the surface of the PCL nanofibers and four types of antifouling polymer brushes were grown by surface-initiated atom transfer radical polymerization (SI-ATRP) from initiator moieties covalently attached to the PDA layer. Cell adhesion was assessed with mouse embryonic fibroblasts (MEFs). MEFs rapidly adhered and formed cell–matrix adhesions (CMAs) with PCL and PCL-PDA nanofibers. Importantly, the nanofibers modified with antifouling polymer brushes were able to suppress non-specific protein adsorption and thereby cell adhesion. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2016
Number of the records: 1