Cryo-SEM and Raman Spectroscopy Study of the Involvement of Polyhydroxyalkanoates in Stress Response of Bacteria

0450102 - ÚPT 2016 RIV US eng J - Journal Article
Krzyžánek, Vladislav - Hrubanová, Kamila - Samek, Ota - Obruča, S. - Márová, I. - Bernatová, Silvie - Šiler, Martin - Zemánek, Pavel
Cryo-SEM and Raman Spectroscopy Study of the Involvement of Polyhydroxyalkanoates in Stress Response of Bacteria.
Microscopy and Microanalysis. Roč. 21, S3 (2015), s. 183-184. ISSN 1431-9276. E-ISSN 1435-8115
R&D Projects: GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01; GA ČR(CZ) GA15-20645S; GA ČR(CZ) GA14-20012S
Institutional support: RVO:68081731
Keywords : cryo-SEM * Raman spectroscopy * polyhydroxyalkanoates
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 1.730, year: 2015

Many bacterial strains are capable of accumulation of very high amounts of polyhydroxyalkanoates (PHAs) in cytoplasm. Consequently, increasing content of the macromolecular material will strongly affect the overall material properties of cells and it is also likely that high content of PHAs inside the cells will also influence the rest of the cell volume – the cytoplasm. PHAs does not serve only as a carbon source in starvation, but presence of PHAs granules in cytoplasm represent additional advantage under stress conditions. It has an impact on metabolic activity of the cells and, moreover, it changes cells morphology as well as physico-chemical properties of the bacterial cytoplasm and cells.
Permanent Link: http://hdl.handle.net/11104/0251507