Nano-colloid printing of functionalized PLA-b-PEO copolymers: tailoring the surface pattern of adhesive motif and its effect on cell attachment

0448568 - ÚMCH 2016 RIV CZ eng J - Journal Article
Mázl Chánová, Eliška - Knotek, P. - Yang, Y. - Machová, Luďka - Proks, Vladimír - Kučka, Jan - Popelka, Štěpán - Pop-Georgievski, Ognen - El Haj, A. - Kubies, Dana - Rypáček, František
Nano-colloid printing of functionalized PLA-b-PEO copolymers: tailoring the surface pattern of adhesive motif and its effect on cell attachment.
Physiological Research. Roč. 64, Suppl. 1 (2015), S61-S73. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA ČR GPP108/12/P624; GA ČR(CZ) GA13-08336S; GA MŠMT(CZ) EE2.3.30.0029; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61389013
Keywords : tissue engineering * surface pattern * AFM
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.643, year: 2015
http://www.biomed.cas.cz/physiolres/pdf/64%20Suppl%201/64_S61.pdf

In this study, we investigate the preparation of surface pattern of functional groups on poly(lactide) (PLA) surfaces through the controlled deposition of core-shell self-assemblies based on functionalized PLA-b-PEO amphiphilic block copolymers from selective solvents. Through grafting RGDS peptide onto the functionalized copolymer surface, the presented approach enables to prepare PLA surfaces with random and clustered spatial distribution of adhesive motifs. The proposed topography of the adhesion motif was proved by atomic force microscopy techniques using biotin-tagged RGDS peptide grafted on the surface and streptavidin-modified gold nanospheres which bind the tagged RGDS peptides as a contrast agent. The cell culture study under static and dynamic conditions with MG63 osteosarcoma cell line showed that the clustered distribution of RGDS peptides provided more efficient initial cell attachment and spreading, and resistance to cell detachment under dynamic culture compared to randomly distributed RGDS motif when with the same average RGDS peptide concentration.
Permanent Link: http://hdl.handle.net/11104/0253904