Mitochondrial ATP synthasome: Expression and structural interaction of its components

0448195 - FGÚ 2016 RIV US eng J - Journal Article
Nůsková, Hana - Mráček, Tomáš - Mikulová, Tereza - Vrbacký, Marek - Kovářová, Nikola - Kovalčíková, Jana - Pecina, Petr - Houštěk, Josef
Mitochondrial ATP synthasome: Expression and structural interaction of its components.
Biochemical and Biophysical Research Communications. Roč. 464, č. 3 (2015), s. 787-793. ISSN 0006-291X. E-ISSN 1090-2104
R&D Projects: GA ČR(CZ) GAP303/12/1363; GA MŠMT(CZ) LL1204
Grant - others:GA UK(CZ) 1160214
Institutional support: RVO:67985823
Keywords : mitochondria * oxidative phosphorylation * supercomplexes * ATP synthasome * ATP synthase
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.371, year: 2015

Mitochondrial ATP synthase, ADP/ATP translocase (ANT), and inorganic phosphate carrier (PiC) are supposed to form a supercomplex called ATP synthasome. Our protein and transcript analysis of rat tissues indicates that the expression of ANT and PiC is transcriptionally controlled in accordance with the biogenesis of ATP synthase. In contrast, the content of ANT and PiC is increased in ATP synthase deficient patients' fibroblasts, likely due to a post-transcriptional adaptive mechanism. A structural analysis of rat heart mitochondria by immunoprecipitation, blue native/SDS electrophoresis, immunodetection and MS analysis revealed the presence of ATP synthasome. However, the majority of PiC and especially ANT did not associate with ATP synthase, suggesting that most of PiC, ANT and ATP synthase exist as separate entities
Permanent Link: http://hdl.handle.net/11104/0249955