Interaction of L-Phenylalanine with a Phospholipid Monolayer at the Water−Air Interface

0446772 - ÚFCH JH 2016 RIV US eng J - Journal Article
Griffith, E. C. - Perkins, R.J. - Telesford, D.-M. - Adams, E. M. - Cwiklik, Lukasz - Allen, H. C. - Roeselová, Martina - Vaida, V.
Interaction of L-Phenylalanine with a Phospholipid Monolayer at the Water−Air Interface.
Journal of Physical Chemistry B. Roč. 119, č. 29 (2015), s. 9038-9048. ISSN 1520-6106. E-ISSN 1520-5207
R&D Projects: GA ČR GA13-06181S
Institutional support: RVO:61388955 ; RVO:61388963
Keywords : SUM-FREQUENCY GENERATION * BREWSTER-ANGLE MICROSCOPY * INTRINSICALLY UNSTRUCTURED PROTEINS
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 3.187, year: 2015

The interaction of L-phenylalanine with a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayer at the air-water interface was explored using a combination of experimental techniques and molecular dynamics (MD) simulations. By means of Langmuir trough methods and Brewster angle microscopy, L-phenylalanine was shown to significantly alter the interfacial tension and the surface domain morphology of the DPPC film. In addition, confocal microscopy was used to explore the aggregation state of L-phenylalanine in the bulk aqueous phase. Finally, MD simulations were performed to gain molecular-level information on the interactions of L-phenylalanine and DPPC at the interface. Taken together, these results show that L-phenylalanine intercalates into a DPPC film at the air-water interface, thereby affecting the surface tension, phase morphology, and ordering of the DPPC film. The results are discussed in the context of biological systems and the mechanism of diseases such as phenylketonuria.
Permanent Link: http://hdl.handle.net/11104/0248742