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Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods
- 1.0440601 - BFÚ 2015 RIV DE eng J - Journal Article
Pilarová, I. - Kejnovská, Iva - Vorlíčková, Michaela - Trnková, L.
Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods.
Electroanalysis. Roč. 26, č. 10 (2014), s. 2118-2128. ISSN 1040-0397. E-ISSN 1521-4109
R&D Projects: GA ČR(CZ) GAP205/12/0466
Institutional support: RVO:68081707
Keywords : DNA heptamers * Voltammetry * Elimination voltammetric procedure
Subject RIV: BO - Biophysics
Impact factor: 2.138, year: 2014
A short, single-stranded DNA fragment d(GCGAAGC) and its analogs d(GCNNNGC) carrying different trinucleotide sequences in the center of the molecule (AAA, CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis). Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stemloop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G-quadruplex is formed.
Permanent Link: http://hdl.handle.net/11104/0243727
Number of the records: 1