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Engineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission
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SYSNO ASEP 0388804 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Engineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission Author(s) Mishra, Kumud (UEK-B) RID, ORCID, SAI
Iannacone, R. (IT)
Petrozza, A. (IT)
Mishra, Anamika (UEK-B) RID
Armentano, N. (IT)
La Vecchia, G. (IT)
Trtílek, M. (CZ)
Cellini, F. (IT)
Nedbal, Ladislav (UEK-B)Source Title Plant Science. - : Elsevier - ISSN 0168-9452
Roč. 182, SI (2012), s. 79-86Number of pages 8 s. Language eng - English Country IE - Ireland Keywords Chlorophyll fluorescence ; Drought ; High-throughput screening ; Solanum lycopersicum ; Transcription factor ; Transgenic plant Subject RIV EH - Ecology, Behaviour R&D Projects OC08055 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 2B06068 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0073 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support RVO:67179843 - RVO:67179843 UT WOS 000298723200010 DOI 10.1016/j.plantsci.2011.03.022 Annotation Drought stress is one of the most important factors that limit crop productivity worldwide. In order to obtain tomato plants with enhanced drought tolerance, we inserted the transcription factor gene ATHB-7 into the tomato genome. This gene was demonstrated earlier to be up-regulated during drought stress in Arabidopsis thaliana thus acting as a negative regulator of growth. We compared the performance of wild type and transgenic tomato line DTL-20, carrying ATHB-7 gene, under well-irrigated and water limited conditions. We found that transgenic plants had reduced stomatal density and stomatal pore size and exhibited an enhanced resistance to soil water deficit. We used the transgenic plants to investigate the potential of chlorophyll fluorescence to report drought tolerance in a simulated high-throughput screening procedure. Wild type and transgenic tomato plants were exposed to drought stress lasting 18 days. The stress was then terminated by rehydration after which recovery was studied for another 2 days. Plant growth, leaf water potential, and chlorophyll fluorescence were measured during the entire experimental period. We found that water potential in wild type and drought tolerant transgenic plants diverged around day 11 of induced drought stress. The chlorophyll fluorescence parameters: the non-photochemical quenching, effective quantum efficiency of PSII, and the maximum quantum yield of PSII photochemistry yielded a good contrast between wild type and transgenic plants from day 7, day 12, and day 14 of induced stress, respectively. We propose that chlorophyll fluorescence emission reports well on the level of water stress and, thus, can be used to identify elevated drought tolerance in high-throughput screens for selection of resistant genotypes. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2013
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