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Modulation of phenotypic and functional maturation of dendritic cells by intestinal bacteria and gliadin: relevance for celiac disease
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SYSNO ASEP 0387751 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Modulation of phenotypic and functional maturation of dendritic cells by intestinal bacteria and gliadin: relevance for celiac disease Author(s) De Palma, G. (ES)
Kamanová, Jana (MBU-M) ORCID, RID
Cinová, Jana (MBU-M)
Olivares, M. (ES)
Drašarová, Hana (MBU-M) RID
Tučková, Ludmila (MBU-M) RID
Sanz, Y. (ES)Source Title Journal of Leukocyte Biology. - : Wiley - ISSN 0741-5400
Roč. 92, č. 5 (2012), s. 1043-1054Number of pages 12 s. Language eng - English Country US - United States Keywords bifidobacteria ; enterobacteria ; cell index Subject RIV EC - Immunology R&D Projects IAA500200801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA310/07/0414 GA ČR - Czech Science Foundation (CSF) GD310/08/H077 GA ČR - Czech Science Foundation (CSF) GA310/09/1640 GA ČR - Czech Science Foundation (CSF) 2B06155 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000310584500015 DOI 10.1189/jlb.111581 Annotation DC maturation and functions are influenced by microbial and environmental stimuli, which could contribute to immune dysfunction. Here, we have investigated the role of enterobacteria ( Escherichia coli CBL2 and Shigella CBD8) isolated from CD patients, bifidobacteria ( Bifidobacterium longum CECT 7347 and Bifidobacterium bifidum CECT 7365), and gliadins on phenotypic and functional features of MDDCs and in coculture with Caco-2 cells. The ultimate goal of our study is to understand the roles played by specific components of the gut microbiota in CD. Enterobacteria induced marked alterations in MDDC morphology, inducing podosome dissolution and dendrites, and activated MDDC adhesion and spreading. Enterobacteria also induced inflammatory cytokine production (IFN-gamma, TNF-alpha, and IL-12), partially resembling the gliadin-induced Th1-type cytokine profile. B. longum CECT 7347 and B. bifidum CECT 7365 induced minor MDDC morphological changes and activated adhesion and spreading and inflammatory cytokine production to a lesser extent compared with enterobacteria. B. longum CECT 7347 also induced lower CD86 and CD40 expression on MDDCs than the two enterobacteria. The aforementioned bifidobacterial strain also reduced gliadin-induced IFN-gamma production and increased IL-10 secretion when both stimuli were combined. Similar trends were detected for MDDCs cocultured with Caco-2 cells. B. longum CECT 7347 reversed the gliadin-reduced ZO-1 expression in Caco-2 cells. Thus, our results suggest that specific components of the gut microbiota may influence phenotypic and functional maturation of DCs differently and their interactions with epithelial cells. This could ultimately define the role of DCs in CD progression Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2013
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