Number of the records: 1  

Comparison of various sample processing methods aiming at improvement of immunogold labeling

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    SYSNO ASEP0371068
    Document TypeA - Abstract
    R&D Document TypeThe record was not marked in the RIV
    R&D Document TypeNení vybrán druh dokumentu
    TitleComparison of various sample processing methods aiming at improvement of immunogold labeling
    Author(s) Sobol, Margaryta (UMG-J) RID
    Philimonenko, Vlada (UMG-J)
    Philimonenko, Anatoly (UMG-J)
    Hozák, Pavel (UMG-J) RID, ORCID
    Source Title3rd High Pressure Freezing Meeting. - Londýn : UCL, London, 2011
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    Number of pages1 s.
    ActionHigh Pressure Freezing Meeting /3./
    Event date13.07.2011-15.07.2011
    VEvent locationLondýn
    CountryGB - United Kingdom
    Event typeEUR
    Languageeng - English
    CountryGB - United Kingdom
    KeywordsHPF ; AFS ; resin embedding ; immunogold
    Subject RIVEB - Genetics ; Molecular Biology
    R&D ProjectsKAN200520704 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    2B06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    CEZAV0Z50520514 - UMG-J (2005-2011)
    AnnotationWe present our experiments aiming to improve the preservation of fine ultrastructural details and antigenicity of biological samples using high-pressure freezing (HPF) followed by automated freeze-substitution (FS) and resin embedment for the subsequent immunogold labeling. We assessed different non-penetrating cryoprotectants such as fetal bovine serum (FBS), low-temperature gelling agarose, and bovine serum albumin (BSA) in various mixtures for HPF to minimize the appearance of segregation patterns and attain a good preservation of an ultrastructure of cellular components, especially nuclei, nucleoli, and mitotic chromatin. We also estimated the ability of various embedding resins LR White, LR Gold, Lowicryl K4M, and Lowicryl HM20 for sensitive revealing the antigens on thin sections by immunogold labeling.
    WorkplaceInstitute of Molecular Genetics
    ContactNikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
    Year of Publishing2012
Number of the records: 1  

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