Boar epididymal fluid: Screening of enzymatic and inhibiting activity

0326014 - BTÚ 2011 CZ eng A - Abstract
Davidová, Nina - Dorosh, Andriy - Jonáková, Věra - Postlerová, Pavla
Boar epididymal fluid: Screening of enzymatic and inhibiting activity.
Sborník - XV. Symposium českých reprodukčních imunologů s mezinárodní účastí. Praha: BTO-N, 2009. s. 34.
[XV. Symposium českých reprodukčních imunologů s mezinárodní účastí. 29.05.2009-31.05.2009, Žďár nad Sázavou]
R&D Projects: GA ČR(CZ) GA523/08/H064; GA MŠMT(CZ) 1M06011; GA ČR(CZ) GA303/09/1285; GA ČR(CZ) GA523/08/HD64
Institutional research plan: CEZ:AV0Z50520701
Keywords : sperm maturation * epididymal fluid * serine proteinase * acrosin inhibitor
Subject RIV: EC - Immunology

Sperm maturation takes place during the transit of spermatozoa through the epididymis. Sperm plasma membrane is exposed to epididymal fluid components essential for maturation. Changes in the sperm membrane proteins are influenced by proteolytic and glycosidic enzymes. Boar epididymal fluid was separated by size exclusion chromatography and by RP HPLC. The SDS-PAGE separated proteins were investigated for enzymatic and proteinase inhibiting activities by substrate and reverse substrate zymographies, respectively. We monitored gene expression and protein changes of inhibitor to acrosin along the epididymis. The presence of acrosin inhibitor of 12 kDa and 8 kDa in epididymal fluid and extract form cauda epididymal spermatozoa was proved using specific polyclonal antibody. Gene expression of acrosin inhibitor increased gradually throughout the epididymal duct, from its entry to the tail.
Permanent Link: http://hdl.handle.net/11104/0173257