Number of the records: 1
Deregulation of acetohydroxy-acid synthase: loss of allosteric inhibition conferred by mutations in the catalytic subunit
- 1.
SYSNO ASEP 0320746 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Deregulation of acetohydroxy-acid synthase: loss of allosteric inhibition conferred by mutations in the catalytic subunit Title Deregulace syntázy kyseliny hydroxyoctové: ztráta alosterické inhibice zapřičiněná mutací v katalytické podjednotce Author(s) Kopecký, Jan (MBU-M)
Kyselková, Martina (MBU-M)
Šigutová, Lucie (MBU-M)
Pospíšil, Stanislav (MBU-M) RID
Felsberg, Jürgen (MBU-M)
Spížek, Jaroslav (MBU-M)
Janata, Jiří (MBU-M) RID, ORCIDSource Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 53, č. 6 (2008), s. 467-471Number of pages 5 s. Language eng - English Country CZ - Czech Republic Keywords acetohydroxy acid ; ilvb genes ; sequencing Subject RIV EE - Microbiology, Virology R&D Projects GA204/01/1001 GA ČR - Czech Science Foundation (CSF) GA204/05/0616 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000262754600001 DOI - Annotation The ilvB genes coding for acetohydroxy acid synthase (AHAS) catalytic subunit from the Streptomyces cinnamonensis parental strain and four mutants deregulated in valine biosynthesis were sequenced. Two changes in the sequence of IlvB conferring the AHAS insensitivity to valine were found in mutant strains: E139A and dQ217. Homology modeling revealed that the substitution E139A is located in a loop at the homodimer subunit-subunit interface near the TPP binding site while dQ217 is situated in a distant helix. Sequencing of ilvBNC upstream region revealed a sequence containing a putative attenuator. However, no mutation within this region was found in mutants with constitutively increased AHAS activity levels suggesting a different mechanism of deregulation Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2009
Number of the records: 1