alpha-Lipoic acid modulates extracellular matrix and angiogenesis gene expression in non-healing wounds treated with hyperbaric oxygen therapy
1.
SYSNO ASEP
0318820
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Článek ve WOS
Title
alpha-Lipoic acid modulates extracellular matrix and angiogenesis gene expression in non-healing wounds treated with hyperbaric oxygen therapy
Title
Kyselina alfa-lipoová moduluje expresi genů pro extracelulární matrix a angiogenezu v nehojících se ranách léčených hyperbarickou kyslíkovou terapií
Author(s)
Alleva, R. (IT) Tomasetti, M. (IT) Sartini, D. (IT) Emanuelli, M. (IT) Nasole, E. (IT) Di Donato, F. (IT) Borghi, B. (IT) Santarelli, L. (IT) Neužil, Jiří (UMG-J)
Source Title
Molecular Medicine
- ISSN 1076-1551
Roč. 14, 3-4 (2008), s. 175-183
alpha-Lipoic acid (LA) has been found previously to accelerate wound repair in patients affected by chronic wounds who underwent hyperbaric oxygen (HBO) therapy. Because proteinases are important in wound repair we hypothesized that LA may regulate matrix metalloproteinase (MMP) expression in cells involved in wound repair. Patients undergoing HBO therapy were double-blind randomized into two groups: LA group and placebo group. Gene expression profiles for MMPs and for angiogenesis mediators were evaluated in biopsies collected at the first HBO session, at the seventh HBO session, and after 14 days of HBO treatment. ELISA tests were used to validate microarray expression of selected genes. LA supplementation in combination with HBO therapy downregulated the inflammatory cytokines and the growth factors which, in turn, affect MMPs expression. The disruption of the positive autocrine feedback loops that maintain the chronic wound state promotes progression of the healing process.
