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Why robust background electrolytes containing multivalent ionic species can fail in capillary zone electrophoresis
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SYSNO ASEP 0162261 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Why robust background electrolytes containing multivalent ionic species can fail in capillary zone electrophoresis Author(s) Beckers, J. L. (NL)
Gebauer, Petr (UIACH-O) RID, ORCID
Boček, Petr (UIACH-O) RIDSource Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 916, č. 1 (2001), s. 41-49Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords capillary zone electrophoresis ; system peaks ; organic acids Subject RIV CB - Analytical Chemistry, Separation R&D Projects IAA4031703 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA4031103 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA203/99/0044 GA ČR - Czech Science Foundation (CSF) GA203/01/0401 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z4031919 - UIACH-O Annotation In this paper it is demonstrated that system peaks are inducted by multivalent weak ionic species in background electrolytes. Their existence is derived from SystCharts and from peak shape diagrams and the theory is confirmed experimentally. If analytes are present in a sample, with mobilities approximately equal to the mobility of a system peak, they interact, resulting in a strong increase of electromigration dispersion. This leads to a strong peak broadening, peak deformation and a loss of resolution. Typical background electrolytes containing multivalent ionic species, e.g. phosphate and phthalate buffers, in literature ofte proposed to be robust electrolytes, are therefore not always universally applicable and can fail for the application of specific analytes. This paper brings a systematic study of the above phenomena and shows theoretical and experimental results for BGEs containing phosphoric acid and phthalic acid. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2002
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