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DNA denaturing capillary electrophoresis in highly alkaline solutions
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SYSNO ASEP 0162214 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title DNA denaturing capillary electrophoresis in highly alkaline solutions Author(s) Klepárník, Karel (UIACH-O) RID, ORCID
Malá, Zdeňka (UIACH-O) RID
Boček, Petr (UIACH-O) RIDSource Title 13th International Symposium on High Performance Capillary Electrophoresis and Related Microscale Techniques. Final Program. Book of Abstracts. - Saarbrücken, 2000 Pages s. 37 Number of pages 1 s. Action International Symposium on High Performance Capillary Electrophoresis and Related Microscale Techniques /13./ Event date 20.02.2000-24.02.2000 VEvent location Saarbrücken Country DE - Germany Language eng - English Country DE - Germany Subject RIV CB - Analytical Chemistry, Separation CEZ AV0Z4031919 - UIACH-O Annotation Denaturing capillary electrophoresis provides much faster and more selective separations of DNA fragments than electrophoresis under native conditions. We have developed a highly alkaline electrolyte system with remarkable properties such as extraordinary denaturing power, high separation and low electroosmotic flow mobility in bare fused-silica capillaries. Thus, the analyses of a microsatellite repeat polymorphism in Endothelin 1 gene under denaturing conditions in short capillaries (2.5 cm) with laser-induced fluorescence detection were accomplished in 42 seconds. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2001
Number of the records: 1