A molecular study of Eubothrium rugosum (Batsch, 1786) (Cestoda: Pseudophyllidea) using ITS rDNA sequences, with notes on the distribution and intraspecific sequence variation of Eubothrium crassum (Bloch, 1779)

0157625 - PAU-O 20033012 RIV DE eng J - Journal Article
Kráľová-Hromadová, I. - Scholz, Tomáš - Shinn, A. - Cunningham, C. - Wootten, R. - Hanzelová, V. - Sommerville, C.
A molecular study of Eubothrium rugosum (Batsch, 1786) (Cestoda: Pseudophyllidea) using ITS rDNA sequences, with notes on the distribution and intraspecific sequence variation of Eubothrium crassum (Bloch, 1779).
Parasitology Research. Roč. 89, č. 6 (2003), s. 473-479. ISSN 0932-0113. E-ISSN 1432-1955
R&D Projects: GA ČR GA524/01/1314
Grant - others:GA SR(SK) VEGA2/1020/21
Institutional research plan: CEZ:AV0Z6022909
Keywords : Cestoda * Eubothrium * ITS rDNA sequence
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 1.000, year: 2003

The internal transcribed spacers (ITS-1 and ITS-2) of the ribosomal DNA (rDNA) of fish cestodes of the genus Eubothrium were sequenced. The ITS sequences of E. rugosum were determined and compared with previously analysed congeneric species, E. crassum and E. salvelini. The ITS-1 sequences of E. rugosum and E. crassum were 535 bp long, the length of E. salvelini ITS-1 was 536 bp. The ITS-2 region was found to be 403 bp in E. rugosum and E. crassum, and 401-402 bp in E. salvelini. The ITS-1 region of E. rugosum was closer to E. salvelini (identity 98.9-99.1%) than to E. crassum (97.9-98.1%), while the sequence similarity within the ITS-2 region was almost identical (97.5-98.0% for E. crassum; 97.7-98.3% for E. salvelini). The intraspecific sequence variation of E. crassum from different fish hosts (freshwater and marine) and European regions was very low, 0.2% for ITS-1 and 0.5% for ITS-2. Analysis of the ITS sequences of specimens from rainbow trout from three localities in Scotland revealed that both E. crassum and E. salvelini are present in this fish host.
Permanent Link: http://hdl.handle.net/11104/0055063