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Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: New perspectives in supported phospholipid bilayer research
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SYSNO ASEP 0043203 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: New perspectives in supported phospholipid bilayer research Title Fluorescenční korelační spektroskopie: Nové perspektivy ve výzkumu fosfolipidových vrstev Author(s) Benda, Aleš (UFCH-W) RID, ORCID
Fagulová, Veronika (UFCH-W)
Deyneka, Alexander (FZU-D)
Enderlain, J. (DE)
Hof, Martin (UFCH-W) RID, ORCIDSource Title Langmuir. - : American Chemical Society - ISSN 0743-7463
Roč. 22, č. 23 (2006), s. 9580-9585Number of pages 7 s. Language eng - English Country US - United States Keywords spectroscopy ; fluorescence ; FLCS Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GA203/05/2308 GA ČR - Czech Science Foundation (CSF) LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40400503 - UFCH-W (2005-2011) AV0Z10100522 - FZU-D (2005-2011) Annotation A new concept based on fluorescence lifetime correlation spectroscopy (FLCS) is presented allowing the simultaneous determination of diffusion coefficients of identical molecules located in different environments. The difference in fluorescence lifetimes, which is the main prerequisite for FLCS, is reached by locating one polution of the dye close to a light-absorbing surface. Since such surfaces quench fluorescence, the fluorescence lifetime of chromophores located close to these surfaces can be tuned in a specific manner. This approach has been demonstrated for a BODIPY-tail-labeled lipid in supported phospholipid bilayers (SPBs) as well as in phospholipid multilayers adsorbed onto solid supports. In particular, the effect of the solid support type on the fluorescence lifetime as well as its dependence on the BODIPY-support distance has been characterized and verified by theoretical considerations based on precise determination of refractive indices of the supports. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2007
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