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A New Method for the Production of High-Concentration Collagen Bioinks with Semiautonomic Preparation
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SYSNO ASEP 0584953 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A New Method for the Production of High-Concentration Collagen Bioinks with Semiautonomic Preparation Author(s) Matějková, J. (CZ)
Kaňoková, D. (CZ)
Šupová, Monika (USMH-B) RID, ORCID, SAI
Matějka, R. (CZ)Number of authors 4 Article number 66 Source Title Gels. - : MDPI
Roč. 10, č. 1 (2024)Number of pages 23 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords bioprinting ; bioink ; collagen hydrogels ; biofabrication ; pH ; neutralization ; automation ; stromal cells OECD category Polymer science R&D Projects NV19-02-00068 GA MZd - Ministry of Health (MZ) Method of publishing Open access Institutional support USMH-B - RVO:67985891 UT WOS 001149227800001 EID SCOPUS 85183415558 DOI 10.3390/gels10010066 Annotation It is believed that 3D bioprinting will greatly help the field of tissue engineering and regenerative medicine, as live patient cells are incorporated into the material, which directly creates a 3D structure. Thus, this method has potential in many types of human body tissues. Collagen provides an advantage, as it is the most common extracellular matrix present in all kinds of tissues and is, therefore, very natural for cells and the organism. Hydrogels with highly concentrated collagen make it possible to create 3D structures without additional additives to crosslink the polymer, which could negatively affect cell proliferation and viability. This study established a new method for preparing highly concentrated collagen bioinks, which does not negatively affect cell proliferation and viability. The method is based on two successive neutralizations of the prepared hydrogel using the bicarbonate buffering mechanisms of the 2× enhanced culture medium and pH adjustment by adding NaOH. Collagen hydrogel was used in concentrations of 20 and 30 mg/mL dissolved in acetic acid with a concentration of 0.05 and 0.1 wt.%. The bioink preparation process is automated, including colorimetric pH detection and adjustment. The new method was validated using bioprinting and subsequent cultivation of collagen hydrogels with incorporated stromal cells. After 96 h of cultivation, cell proliferation and viability were not statistically significantly reduced. Workplace Institute of Rock Structure and Mechanics Contact Iva Švihálková, svihalkova@irsm.cas.cz, Tel.: 266 009 216 Year of Publishing 2025 Electronic address https://doi.org/10.3390/gels10010066
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