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Spatial organization and dynamics of chromosomes and microtubules during barley mitosis
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SYSNO ASEP 0575548 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Spatial organization and dynamics of chromosomes and microtubules during barley mitosis Author(s) Kaduchová, Kateřina (UEB-Q) ORCID
Marchetti, C. (GB)
Ovečka, M. (CZ)
Galuszka, P. (CZ)
Bergougnoux, V. (CZ)
Šamaj, J. (CZ)
Pečinka, Aleš (UEB-Q) ORCID, RIDNumber of authors 7 Source Title Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 115, č. 3 (2023), s. 602-613Number of pages 12 s. Language eng - English Country US - United States Keywords 3D nuclear organization ; barley ; chromatin ; chromosomes ; confocal microscopy ; crops ; Hordeum vulgare ; live imaging ; microtubules ; mitosis OECD category Cell biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC18026 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA21-02929S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 001022301200001 EID SCOPUS 85164109102 DOI 10.1111/tpj.16355 Annotation Mitosis and cytokinesis are fundamental processes through which somatic cells increase their numbers and allow plant growth and development. Here, we analyzed the organization and dynamics of mitotic chromosomes, nucleoli, and microtubules in living cells of barley root primary meristems using a series of newly developed stable fluorescent protein translational fusion lines and time-lapse confocal microscopy. The median duration of mitosis from prophase until the end of telophase was 65.2 and 78.2 min until the end of cytokinesis. We showed that barley chromosomes frequently start condensation before mitotic pre-prophase as defined by the organization of microtubules and maintain it even after entering into the new interphase. Furthermore, we found that the process of chromosome condensation does not finish at metaphase, but gradually continues until the end of mitosis. In summary, our study features resources for in vivo analysis of barley nuclei and chromosomes and their dynamics during mitotic cell cycle. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2024 Electronic address https://doi.org/10.1111/tpj.16355
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