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110 μm thin endo-microscope for deep-brain in vivo observations of neuronal connectivity, activity and blood flow dynamics
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SYSNO ASEP 0571074 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title 110 μm thin endo-microscope for deep-brain in vivo observations of neuronal connectivity, activity and blood flow dynamics Author(s) Stibůrek, Miroslav (UPT-D)
Ondráčková, Petra (UPT-D)
Tučková, Tereza (UPT-D) ORCID
Turtaev, S. (DE)
Šiler, Martin (UPT-D) RID, ORCID, SAI
Pikálek, Tomáš (UPT-D) RID, ORCID, SAI
Jákl, Petr (UPT-D) RID, ORCID, SAI
Gomes, A. D. (DE)
Krejčí, Jana (BFU-R) RID, ORCID
Kolbábková, Petra (UPT-D)
Uhlířová, Hana (UPT-D) ORCID, RID
Čižmár, Tomáš (UPT-D) RID, ORCID, SAINumber of authors 12 Article number 1897 Source Title Nature Communications. - : Nature Publishing Group
Roč. 14, č. 1 (2023)Number of pages 9 s. Publication form Online - E Language eng - English Country US - United States Keywords holographic endoscopy ; multi-mode fibre ; in vivo ; imaging ; brain ; mouse ; calcium imaging ; blood flow Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) Subject RIV - cooperation Institute of Biophysics - Cell Biology R&D Projects EF15_003/0000476 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support UPT-D - RVO:68081731 ; BFU-R - RVO:68081707 UT WOS 000964899900010 EID SCOPUS 85151785916 DOI 10.1038/s41467-023-36889-z Annotation Light-based in-vivo brain imaging relies on light transport over large distances of highly scattering tissues. Scattering gradually reduces imaging contrast and resolution, making it difficult to reach structures at greater depths even with the use of multiphoton techniques. To reach deeper, minimally invasive endo-microscopy techniques have been established. These most commonly exploit graded-index rod lenses and enable a variety of modalities in head-fixed and freely moving animals. A recently proposed alternative is the use of holographic control of light transport through multimode optical fibres promising much less traumatic application and superior imaging performance. We present a 110 μm thin laser-scanning endo-microscope based on this prospect, enabling in-vivo volumetric imaging throughout the whole depth of the mouse brain. The instrument is equipped with multi-wavelength detection and three-dimensional random access options, and it performs at lateral resolution below 1 μm. We showcase various modes of its application through the observations of fluorescently labelled neurones, their processes and blood vessels. Finally, we demonstrate how to exploit the instrument to monitor calcium signalling of neurones and to measure blood flow velocity in individual vessels at high speeds. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2024 Electronic address https://www.nature.com/articles/s41467-023-36889-z
Number of the records: 1