Number of the records: 1  

Combined in vitro and cell-based selection display method producing specific binders against IL-9 receptor in high yields

  1. 1.
    SYSNO ASEP0569019
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleCombined in vitro and cell-based selection display method producing specific binders against IL-9 receptor in high yields
    Author(s) Huličiak, Maroš (BTO-N)
    Biedermannová, Lada (BTO-N) RID, ORCID
    Berdár, Daniel (BTO-N)
    Herynek, Štěpán (BTO-N)
    Kolářová, Lucie (BTO-N)
    Tomala, Jakub (BTO-N)
    Mikulecký, Pavel (BTO-N) RID
    Schneider, Bohdan (BTO-N) RID, ORCID
    Number of authors8
    Source TitleFEBS Journal - ISSN 1742-464X
    Roč. 290, č. 11 (2023), s. 2993-3005
    Number of pages13 s.
    Languageeng - English
    CountryGB - United Kingdom
    Keywordsdirected evolution ; interleukin 9 receptor alpha ; protein scaffolds ; ribosome display ; yeast display
    Subject RIVEB - Genetics ; Molecular Biology
    OECD categoryBiochemistry and molecular biology
    R&D ProjectsLX22NPO5102 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    LM2018127 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    EF18_046/0015974 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    GA20-13029S GA ČR - Czech Science Foundation (CSF)
    Method of publishingOpen access
    Institutional supportBTO-N - RVO:86652036
    UT WOS000919058700001
    EID SCOPUS85147001025
    DOI10.1111/febs.16726
    AnnotationWe combined cell-free ribosome display and cell-based yeast display selection to build specific protein binders to the extracellular domain of the human interleukin 9 receptor alpha (IL-9Rα). The target, IL-9Rα, is the receptor involved in the signalling pathway of IL-9, a pro-inflammatory cytokine medically important for its involvement in respiratory diseases. The successive use of modified protocols of ribosome and yeast displays allowed us to combine their strengths—the virtually infinite selection power of ribosome display and the production of (mostly) properly folded and soluble proteins in yeast display. The described experimental protocol is optimized to produce binders highly specific to the target, including selectivity to common proteins such as BSA, and proteins potentially competing for the binder such as receptors of other cytokines. The binders were trained from DNA libraries of two protein scaffolds called 57aBi and 57bBi developed in our laboratory. We show that the described unconventional combination of ribosome and yeast displays is effective in developing selective small protein binders to the medically relevant molecular target.
    WorkplaceInstitute of Biotechnology
    ContactMonika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
    Year of Publishing2024
    Electronic addresshttps://febs.onlinelibrary.wiley.com/doi/10.1111/febs.16726
Number of the records: 1  

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