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Silver Nanoparticles Alter Microtubule Arrangement, Dynamics and Stress Phytohormone Levels
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SYSNO ASEP 0563305 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Silver Nanoparticles Alter Microtubule Arrangement, Dynamics and Stress Phytohormone Levels Author(s) Angelini, J. (CZ)
Klassen, R. (CZ)
Široká, Jitka (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Záruba, K. (CZ)
Siegel, J. (CZ)
Novotná, Z. (CZ)
Valentová, O. (CZ)Number of authors 8 Article number 313 Source Title Plants. - : MDPI
Roč. 11, č. 3 (2022)Number of pages 26 s. Language eng - English Country CH - Switzerland Keywords Abscisic acid ; Arabidopsis thaliana cotyledon ; FRAP method ; Gl-1 mutant ; Jasmonic acid ; Microtubular dynamics ; Microtubular pattern ; Silver ion ; Silver nanoparticles ; Stress phytohormones OECD category Plant sciences, botany R&D Projects GA17-10907S GA ČR - Czech Science Foundation (CSF) EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000759440400001 EID SCOPUS 85123343393 DOI 10.3390/plants11030313 Annotation The superior properties of silver nanoparticles (AgNPs) has resulted in their broad utilization worldwide, but also the risk of irreversible environment infestation. The plant cuticle and cell wall can trap a large part of the nanoparticles and thus protect the internal cell structures, where the cytoskeleton, for example, reacts very quickly to the threat, and defense signaling is subsequently triggered. We therefore used not only wild-type Arabidopsis seedlings, but also the glabra 1 mutant, which has a different composition of the cuticle. Both lines had GFP-labeled microtubules (MTs), al-lowing us to observe their arrangement. To quantify MT dynamics, we developed a new microscopic method based on the FRAP technique. The number and growth rate of MTs decreased significantly after AgNPs, similarly in both lines. However, the layer above the plasma membrane thickened significantly in wild-type plants. The levels of three major stress phytohormone derivatives—jasmonic, abscisic, and salicylic acids—after AgNP (with concomitant Ag+) treatment increased significantly (particularly in mutant plants) and to some extent resembled the plant response after mechanical stress. The profile of phytohormones helped us to estimate the mechanism of response to AgNPs and also to understand the broader physiological context of the observed changes in MT structure and dynamics. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address https://doi.org/10.3390/plants11030313
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