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Mutation in Drosophila concentrative nukleoside transporter 1 alters spermatid maturation and mating behavior
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SYSNO ASEP 0560627 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mutation in Drosophila concentrative nukleoside transporter 1 alters spermatid maturation and mating behavior Author(s) Maaroufi, Houda Ouns (BC-A) ORCID
Pauchová, Lucie (BC-A)
Lin, Yu-Hsien (BC-A) ORCID
Wu, Chia-hsiang (BC-A)
Rouhová, Lenka (BC-A) ORCID
Kučerová, Lucie (BC-A) RID, ORCID
Vieira, Lígia Cota (BC-A)
Renner, M. (CZ)
Sehadová, Hana (BC-A) RID, ORCID
Hradilová, Miluše (UMG-J)
Žurovec, Michal (BC-A) RID, ORCIDNumber of authors 11 Article number 945572 Source Title Frontiers in Cell and Developmental Biology. - : Frontiers Research Foundation - ISSN 2296-634X
Roč. 10, AUG 23 (2022)Number of pages 17 s. Language eng - English Country CH - Switzerland Keywords cnt1 ; gamete ; spermatogenesis Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology Subject RIV - cooperation Institute of Molecular Genetics - Genetics ; Molecular Biology R&D Projects GJ19-13784Y GA ČR - Czech Science Foundation (CSF) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 ; UMG-J - RVO:68378050 UT WOS 000853224400001 EID SCOPUS 85138255369 DOI 10.3389/fcell.2022.945572 Annotation Concentrative nucleoside transporters (Cnts) are unidirectional carriers that mediate the energy-costly influx of nucleosides driven by the transmembrane sodium gradient. Cnts are transmembrane proteins that share a common structural organization and are found in all phyla. Although there have been studies on Cnts from a biochemical perspective, no deep research has examined their role at the organismal level. Here, we investigated the role of the Drosophila melanogaster cnt1 gene, which is specifically expressed in the testes. We used the CRISPR/Cas9 system to generate a mutation in the cnt1 gene. The cnt1 mutants exhibited defects in the duration of copulation and spermatid maturation, which significantly impaired male fertility. The most striking effect of the cnt1 mutation in spermatid maturation was an abnormal structure of the sperm tail, in which the formation of major and minor mitochondrial derivatives was disrupted. Our results demonstrate the importance of cnt1 in male fertility and suggest that the observed defects in mating behavior and spermatogenesis are due to alterations in nucleoside transport and associated metabolic pathways. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2023 Electronic address https://www.frontiersin.org/articles/10.3389/fcell.2022.945572/pdf
Number of the records: 1