Methods of in vitro study of galectin-glycomaterial interaction

0559850 - MBÚ 2023 RIV US eng J - Journal Article
Heine, Viktoria - Dey, C. - Bojarová, Pavla - Křen, Vladimír - Elling, L.
Methods of in vitro study of galectin-glycomaterial interaction.
Biotechnology Advances. Roč. 58, SEP 2022 (2022), č. článku 107928. ISSN 0734-9750. E-ISSN 1873-1899
R&D Projects: GA ČR(CZ) GA20-00215S; GA MŠMT(CZ) LTC20069; GA MŠMT(CZ) LTC18041; GA MŠMT(CZ) LTC20072; EC(CZ) CA18132
EU Projects: European Commission(XE) CA18103; European Commission(XE) CA16225; European Commission(CZ) CA16122 - BIONECA
Grant - others:AV ČR(CZ) DAAD-20-04
Program: Bilaterální spolupráce
Institutional support: RVO:61388971
Keywords : Biosensor * Carbohydrate * Cancer * Galectin * Glycoconjugate * Inflammation
OECD category: Biochemistry and molecular biology
Impact factor: 16, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0734975022000246?via%3Dihub

Galectins are a family of carbohydrate-binding lectins modulating cell events such as cell proliferation, apoptosis, adhesion or migration by cross-linking the glycan structures of cell membranes and/or extracellular matrix components. In a diseased organism, galectins are upregulated and trigger the progression of diseases such as inflammation, cancerogenesis, fibrosis, cardiovascular and metabolic disorders. Targeting galectins with glycomaterials for the aims of diagnostics or therapy is, therefore, a focus of biotechnological and biomedicinal research, and already led to candidates for clinical trials. Testing and evaluation of galectin-glycomaterial interactions require informative and versatile analytical methods at several levels of knowledge, from basic intermolecular interaction to complex cell-based assays. This review aims to classify and characterize a selection of the most promising methods to identify the prospective glycomaterials for translating galectin targeting from the molecular level to the level of tailored in vivo assays.
Permanent Link: https://hdl.handle.net/11104/0333249