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Detailed Characteristics of Tonsillar Tumors with Extrachromosomal or Integrated Form of Human Papillomavirus
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SYSNO ASEP 0559716 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Detailed Characteristics of Tonsillar Tumors with Extrachromosomal or Integrated Form of Human Papillomavirus Author(s) Pokryvkova, B. (CZ)
Salaková, M. (CZ)
Šmahelová, J. (CZ)
Vojtěchová, Z. (CZ)
Novosadová, Vendula (UMG-J)
Tachezy, R. (CZ)Number of authors 6 Article number 42 Source Title Viruses. - : MDPI
Roč. 12, č. 1 (2020)Number of pages 16 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords human papillomavirus ; E2 binding sites ; methylation ; genome status Subject RIV EB - Genetics ; Molecular Biology OECD category Virology R&D Projects LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000785 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 000515930700053 DOI 10.3390/v12010042 Annotation The human papillomavirus (HPV) integration, the critical step in viral carcinogenesis, most frequently occurs in the E2 gene, which results in its inactivation and in an increase of E6/E7 transcription. However, in a substantial number of tumors, the virus is present in an extrachromosomal form. For those tumors, the transformation mechanisms are not fully elucidated. Here we evaluated the possible mechanism of inactivating the E2 without interruption of the gene, methylation or mutation of the E2 binding sites (E2BSs) in HPV16-positive tonsillar tumors by next-generation and Sanger sequencing. Viral genome status was analyzed by the amplification of papillomavirus oncogene transcripts assay (APOT) and mRNA mapping, and expression of viral oncogenes was performed by qPCR. The methylation of E2BSs was significantly higher in tumors with an integrated, in comparison to extrachromosomal, form of the viral genome. No mutations were detected in the E2BSs. The viral oncogenes were equally expressed in samples with an integrated and extrachromosomal form of the virus. Only the nucleotide variants were identified in the E2 gene. No proposed mechanism of E2 inactivation was confirmed in tonsillar tumors with an extrachromosomal form of the HPV genome. The expression of E6/E7 genes seems to be sufficient to initiate and maintain the carcinogenic process Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2023 Electronic address https://www.mdpi.com/1999-4915/12/1/42
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