Number of the records: 1
An ABC transporter Wzm-Wzt catalyzes translocation of lipid-linked galactan across the plasma membrane in mycobacteria
- 1.
SYSNO ASEP 0554945 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title An ABC transporter Wzm-Wzt catalyzes translocation of lipid-linked galactan across the plasma membrane in mycobacteria Author(s) Savková, K. (SK)
Huszár, S. (SK)
Baráth, P. (SK)
Pakanová, Z. (SK)
Kozmon, S. (SK)
Vancová, Marie (BC-A) RID, ORCID
Tesařová, Martina (BC-A) ORCID
Blasko, J. (SK)
Kalinak, M. (SK)
Singh, V. (ZA)
Korduláková, J. (SK)
Mikušová, K. (SK)Number of authors 12 Article number e2023663118 Source Title Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 118, č. 17 (2021)Number of pages 10 s. Publication form Online - E Language eng - English Country US - United States Keywords lipopolysaccharide o-antigens ; cell-wall ; klebsiella-pneumoniae ; escherichia-coli ; linkage region ; chain-length ; biosynthesis ; arabinogalactan ; tuberculosis ; lipoarabinomannan ; mycobacterium ; cell wall ; transport ; galactan Subject RIV EA - Cell Biology OECD category Cell biology R&D Projects LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000648438500019 EID SCOPUS 85105348097 DOI 10.1073/pnas.2023663118 Annotation Mycobacterium tuberculosis, one of the deadliest pathogens in human history, is distinguished by a unique, multilayered cell wall, which offers the bacterium a high level of protection from the attacks of the host immune system. The primary structure of the cell wall core, composed of covalently linked peptidoglycan, branched heteropolysaccharide arabinogalactan, and mycolic acids, is well known, and numerous enzymes involved in the biosynthesis of its components are characterized. The cell wall biogenesis takes place at both cytoplasmic and periplasmic faces of the plasma membrane, and only recently some of the specific transport systems translocating the metabolic intermediates between these two compartments have been characterized [M. Jackson, C. M. Stevens, L. Zhang, H. I. Zgurskaya, M. Niederweis, Chem. Rev., 10.1021/acs.chemrev.0c00869 (2020)]. In this work, we use CRISPR interference methodology in Mycobacterium smegmatis to functionally characterize an ATP binding cassette (ABC) transporter involved in the translocation of galactan precursors across the plasma membrane. We show that genetic knockdown of the transmembrane subunit of the transporter results in severe morphological changes and the accumulation of an aberrantly long galactan precursor. Based on similarities with structures and functions of specific O-antigen ABC transporters of gram-negative bacteria [C. Whitfield, D. M. Williams, S. D. Kelly, J. Biol. Chem. 295, 10593-10609 (2020)], we propose a model for coupled synthesis and export of the galactan polymer precursor in mycobacteria. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2022 Electronic address https://www.pnas.org/doi/full/10.1073/pnas.2023663118
Number of the records: 1