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Palmitoylated Prolactin-releasing Peptide Reduced Aβ Plaques and Microgliosis in the Cerebellum: APP/PS1 Mice Study
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SYSNO ASEP 0551939 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Palmitoylated Prolactin-releasing Peptide Reduced Aβ Plaques and Microgliosis in the Cerebellum: APP/PS1 Mice Study Author(s) Mengr, A. (CZ)
Hrubá, L. (CZ)
Exnerová, A. (CZ)
Holubová, M. (CZ)
Popelová, A. (CZ)
Železná, B. (CZ)
Kuneš, Jaroslav (FGU-C) RID, ORCID
Maletínská, L. (CZ)Source Title Current Alzheimer Research. - : Bentham Science Publishers - ISSN 1567-2050
Roč. 18, č. 8 (2021), s. 607-622Number of pages 16 s. Language eng - English Country NL - Netherlands Keywords APP/PS1 mice ; Alzheimer´s disease ; palm11-PrRP31 ; hippocampus ; cerebellum ; amyloid-beta plaques ; neuroinflammation ; synaptogenesis OECD category Physiology (including cytology) R&D Projects GA20-00546S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000723717100001 EID SCOPUS 85125720272 DOI 10.2174/1567205018666210922110652 Annotation Background: Prolactin-releasing peptide (PrRP) is a potential drug for the treatment of obesity and associated Type 2 Diabetes Mellitus (T2DM) due to its strong anorexigenic and antidiabetic properties. In our recent study, the lipidized PrRP analog palm(II)-PrRP31 was proven to exert beneficial effects in APP/PS1 mice, a model of Alzheimer's Disease (AD)-like amyloid-beta (A beta) pathology, reducing the A beta plaque load, microgliosis and astrocytosis in the hippocampus and cortex.
Objective: In this study, we focused on the neuroprotective and anti-inflammatory effects of palm(11)-PrRP31 and its possible impact on synaptogenesis in the cerebellum of APP/PS1 mice, be cause others have suggested that cerebellar A beta plaques contribute to cognitive deficits in AD.
Methods: APP/PS1 mice were treated subcutaneously with palm(11)-PrRP31 for 2 months, then immunoblotting and immunohistochemistry were used to quantify pathological markers connected to AD, compared to control mice.
Results: In the cerebella of 8 months old APP/PS1 mice, we found widespread A beta plaques surrounded by activated microglia detected by ionized calcium-binding adapter molecule (Iba1), but no increase in astrocytic marker Glial Fibrillary Acidic Protein (GFAP) compared to controls. Interestingly, no difference in both presynaptic markers syntaxin1A and postsynaptic marker spinophilin was registered between APP/PS1 and control mice. Palm(11)-PrRP31 treatment significantly reduced the A beta plaque load and microgliosis in the cerebellum. Furthermore, palm(11)-PrRP31 increased synaptogenesis and attenuated neuroinflammation and apoptosis in the hippocampus of APP/PS1 mice.
Conclusion: These results suggest palm(11)-PrRP31 is a promising agent for the treatment of neurodegenerative disorders.Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://dx.doi.org/10.2174/1567205018666210922110652
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