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Limited light intensity and low temperature: Can plants survive freezing in light conditions that more accurately replicate the cold season in temperate regions?
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SYSNO ASEP 0551151 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Limited light intensity and low temperature: Can plants survive freezing in light conditions that more accurately replicate the cold season in temperate regions? Author(s) Novák, J. (CZ)
Černý, M. (CZ)
Roignant, J. (CZ)
Skalák, J. (CZ)
Saiz-Fernández, I. (CZ)
Luklová, M. (CZ)
Skaláková, P. (CZ)
Ondrisková, V. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Tarkowská, Danuše (UEB-Q) RID, ORCID
Kameniarová, M. (CZ)
Karady, Michal (UEB-Q) ORCID
Vaňková, Radomíra (UEB-Q) RID, ORCID
Brzobohatý, Břetislav (BFU-R) RID, ORCIDNumber of authors 15 Article number 104581 Source Title Environmental and Experimental Botany. - : Elsevier - ISSN 0098-8472
Roč. 190, OCT (2021)Number of pages 13 s. Language eng - English Country NL - Netherlands Keywords Acclimation ; Arabidopsis thaliana ; Cold ; Cytokinin ; Freezing stress ; Light ; Low PPFD ; Metabolome ; Proteome OECD category Plant sciences, botany R&D Projects GA17-04607S GA ČR - Czech Science Foundation (CSF) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LQ1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 ; BFU-R - RVO:68081707 UT WOS 000685009900002 EID SCOPUS 85111347020 DOI 10.1016/j.envexpbot.2021.104581 Annotation Plants in temperate regions have evolved mechanisms that enable them to survive sudden temperature drops. Experiments with plants grown in long-day conditions, in which they are most sensitive to freezing stress, indicate that the cold acclimation mechanism is light-dependent and does not fully operate under low light intensity. However, winter annuals like Arabidopsis thaliana Col-0 germinate in the fall, overwinter as rosettes, and thus must acclimate under short photoperiods and low irradiance. Thus, we have analysed effects of variations in light intensity in plants grown under short-day photoperiod at the 1.14 growth stage (14 rosette leaves). Plants were acclimated at 4 °C for seven days under control and limited-light conditions: 100 and 20 μmol m-2s-1 photosynthetic photon flux density (PPFD), respectively. All cold-acclimated plants accumulated molecular markers reportedly associated with acquired freezing tolerance, including proline, sucrose, cold-responsive gene transcripts, dehydrins and low temperature-induced proteins. Observed changes (and similarity of freezing stress survival rates of plants in both light conditions) indicate that low PPFD did not inhibit the cold acclimation process. The molecular analysis identified distinct PPFD-specific adaptation mechanisms manifested in contrasting contents of anthocyanins, cytokinin conjugates, photosystem proteins, and enzymes involved in protein, energy, and reactive oxygen species metabolism. Finally, the results identify putative proteins and metabolite markers correlating with susceptibility to freezing stress of non-acclimated plants grown under low PPFD. Our data show that Arabidopsis plants grown under short-day photoperiods can be fully cold-acclimated under limited light conditions, employing standard and PPFD-specific pathways. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1016/j.envexpbot.2021.104581
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