Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

Sedláček, I.; Holochová, P.; Sobotka, Roman; Busse, H.-J.; Švec, P.; Králová, S.; Šedo, O.; Pilný, Jan; Staňková, E.; Koublová, V.; Sedlář, K.

doi:https://dx.doi.org/10.1128/Spectrum.00452-21

Number of the records: 1

Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.

1.

SYSNO ASEP	0550061
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Classification of a Violacein-Producing Psychrophilic Group of Isolates Associated with Freshwater in Antarctica and Description of Rugamonas violacea sp. nov.
Author(s)	Sedláček, I. (CZ) Holochová, P. (CZ) Sobotka, Roman (MBU-M)_{RID, ORCID} Busse, H.-J. (AT) Švec, P. (CZ) Králová, S. (CZ) Šedo, O. (CZ) Pilný, Jan (MBU-M)_ORCID Staňková, E. (CZ) Koublová, V. (CZ) Sedlář, K. (CZ)
Article number	e00452-21
Source Title	Microbiology Spectrum. - : American Society for Microbiology - ISSN 2165-0497 Roč. 9, č. 1 (2021)
Number of pages	14 s.
Language	eng - English
Country	US - United States
Keywords	psychrophiles ; Rugamonas ; taxonomy ; Antarctica ; violacein ; species description
Subject RIV	EE - Microbiology, Virology
OECD category	Microbiology
Research Infrastructure	CzechPolar2 - 90078 - Masarykova univerzita CIISB II - 90127 - Masarykova univerzita
Method of publishing	Open access
Institutional support	MBU-M - RVO:61388971
UT WOS	000703076500085
EID SCOPUS	85115896136
DOI	10.1128/Spectrum.00452-21
Annotation	A group of 11 bacterial strains was isolated from streams and lakes located in a deglaciated northern part of James Ross Island, Antarctica. They were rod-shaped, Gram-stain-negative, motile, and catalase-positive and produced blue-violet-pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, automated ribotyping, repetitive element sequence-based PCR (rep-PCR), MALDI-TOF MS, fatty acid profile, chemotaxonomy analyses, and extensive biotyping was applied in order to clarify the taxonomic position of these isolates. Phylogenetic analysis based on the 16S rRNA gene indicated that all the isolates constituted a coherent group belonging to the genus Rugamonas. The closest relatives to the representative isolate P5900(T) were Rugamonas rubra CCM 3730(T), Rugamonas rivuli FT103W(T), and Rugamonas aquatica FT29W(T), exhibiting 99.2%, 99.1%, and 98.6% 16S rRNA pairwise similarity, respectively. The average nucleotide identity and digital DNA-DNA hybridization values calculated from the whole-genome sequencing data clearly proved that P5900(T) represents a distinct Rugamonas species. The G+C content of genomic DNAs was 66.1 mol%. The major components in fatty acid profiles were summed feature 3 (C-16:1 omega 7c/C-16:1 omega 6c), C-16:0, and C-12:0. The cellular quinone content contained exclusively ubiquinone Q-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The polyamine pattern was composed of putrescine, 2-hydroxputrescine, and spermidine.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2022
Electronic address	https://journals.asm.org/doi/10.1128/Spectrum.00452-21

Number of the records: 1