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Developmental Changes in Peripherin-eGFP Expression in Spiral Ganglion Neurons
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SYSNO ASEP 0548736 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Developmental Changes in Peripherin-eGFP Expression in Spiral Ganglion Neurons Author(s) Elliott, K. L. (US)
Kersigo, J. (US)
Lee, J. H. (US)
Jahan, I. (US)
Pavlínková, Gabriela (BTO-N) RID, ORCID
Fritzsch, B. (US)
Yamoah, E. N. (US)Number of authors 7 Source Title Frontiers in Cellular Neuroscience. - : Frontiers Media
Roč. 15, JUN 15 2021 (2021)Number of pages 12 s. Language eng - English Country US - United States Keywords peripherin ; Prph-eGFP ; type II spiral ganglion neurons Subject RIV FH - Neurology OECD category Neurosciences (including psychophysiology R&D Projects GA20-06927S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support BTO-N - RVO:86652036 UT WOS 000667688500001 EID SCOPUS 85109015187 DOI 10.3389/fncel.2021.678113 Annotation The two types of spiral ganglion neurons (SGNs), types I and II, innervate inner hair cells and outer hair cells, respectively, within the mammalian cochlea and send another process back to cochlear nuclei in the hindbrain. Studying these two neuronal types has been made easier with the identification of unique molecular markers. One of these markers, peripherin, was shown using antibodies to be present in all SGNs initially but becomes specific to type II SGNs during maturation. We used mice with fluorescently labeled peripherin (Prph-eGFP) to examine peripherin expression in SGNs during development and in aged mice. Using these mice, we confirm the initial expression of Prph-eGFP in both types I and II neurons and eventual restriction to only type II perikarya shortly after birth. However, while Prph-eGFP is uniquely expressed within type II cell bodies by P8, both types I and II peripheral and central processes continue to express Prph-eGFP for some time before becoming downregulated. Only at P30 was there selective type II Prph-eGFP expression in central but not peripheral processes. By 9 months, only the type II cell bodies and more distal central processes retain Prph-eGFP expression. Our results show that Prph-eGFP is a reliable marker for type II SGN cell bodies beyond P8, however, it is not generally a suitable marker for type II processes, except for central processes beyond P30. How the changes in Prph-eGFP expression relate to subsequent protein expression remains to be explored. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2022 Electronic address https://www.frontiersin.org/articles/10.3389/fncel.2021.678113/full
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