The role of the biotin linker in polymer antibody mimetics, iBodies, in biochemical assays

Šubr, Vladimír; Ormsby, Tereza; Šácha, Pavel; Konvalinka, Jan; Etrych, Tomáš; Kostka, Libor

doi:https://dx.doi.org/10.1039/d1py00707f

Number of the records: 1

The role of the biotin linker in polymer antibody mimetics, iBodies, in biochemical assays

1.

SYSNO ASEP	0547173
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The role of the biotin linker in polymer antibody mimetics, iBodies, in biochemical assays
Author(s)	Šubr, Vladimír (UMCH-V)_{RID, ORCID} Ormsby, Tereza (UOCHB-X)_{ORCID, RID} Šácha, Pavel (UOCHB-X)_{RID, ORCID} Konvalinka, Jan (UOCHB-X)_{RID, ORCID} Etrych, Tomáš (UMCH-V)_{RID, ORCID} Kostka, Libor (UMCH-V)_{RID, ORCID}
Source Title	Polymer Chemistry . - : Royal Society of Chemistry - ISSN 1759-9954 Roč. 12, č. 41 (2021), s. 6009-6021
Number of pages	13 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	HPMA ; iBody ; biotin
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
Subject RIV - cooperation	Institute of Organic Chemistry and Biochemistry - Biochemistry
R&D Projects	GA19-05649S GA ČR - Czech Science Foundation (CSF)
	GA19-10280S GA ČR - Czech Science Foundation (CSF)
	EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	UMCH-V - RVO:61389013 ; UOCHB-X - RVO:61388963
UT WOS	000705635600001
EID SCOPUS	85118290455
DOI	10.1039/d1py00707f
Annotation	Recently, we have developed synthetic polymer-based antibody mimetics called iBodies that can successfully replace antibodies in many biochemical applications including ELISA (enzyme-linked immunosorbent assay), flow cytometry, immunocytochemistry, affinity purification, and confocal microscopy. In this study, we focused primarily on the effect of a linker between the polymer backbone and biotin, and then on the influence of the number of biotins per polymer chain on the efficacy of the ELISA or pull-down assays. In addition, the biotin position on the polymer chain was investigated. Moreover, we developed a novel chain transfer agent suitable for RAFT polymerization, which enables the functionalization of specifically the polymer precursors and simplifies the synthesis of semitelechelic antibody mimetic materials. By employing optimized iBodies the sensitivity of the ELISA with a lengthened linker between the polymer backbone and biotin was increased up to 5 times. Importantly, we found that one biotin at the end of the polymer chain can replace up to 12 biotins located along the polymer chain and maintain the signal level in the ELISA, as well as in the pull-down assay.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2022
Electronic address	https://pubs.rsc.org/en/content/articlelanding/2021/PY/D1PY00707F

Number of the records: 1