Number of the records: 1
The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization
- 1.0545891 - ÚOCHB 2022 RIV GB eng J - Journal Article
Doron-Mandel, E. - Koppel, I. - Abraham, O. - Rishal, I. - Smith, T. P. - Buchanan, C. N. - Sahoo, P. K. - Kadlec, Jan - Oses-Prieto, J. A. - Kawaguchi, R. - Alber, S. - Zahavi, E. E. - Di Matteo, P. - Di Pizio, A. - Song, D. A. - Okladnikov, N. - Gordon, D. - Ben-Dor, S. - Haffner-Krausz, R. - Coppola, G. - Burlingame, A. L. - Jungwirth, Pavel - Twiss, J. L. - Fainzilber, M.
The glycine arginine-rich domain of the RNA-binding protein nucleolin regulates its subcellular localization.
EMBO Journal. Roč. 40, č. 20 (2021), č. článku e107158. ISSN 0261-4189. E-ISSN 1460-2075
R&D Projects: GA ČR(CZ) GX19-26854X
Institutional support: RVO:61388963
Keywords : axonal transport * cell size regulation * local translation * protein–membrane interaction * subcellular localization
OECD category: Biophysics
Impact factor: 13.783, year: 2021
Method of publishing: Open access
https://doi.org/10.15252/embj.2020107158
Nucleolin is a multifunctional RNA Binding Protein (RBP) with diverse subcellular localizations, including the nucleolus in all eukaryotic cells, the plasma membrane in tumor cells, and the axon in neurons. Here we show that the glycine arginine rich (GAR) domain of nucleolin drives subcellular localization via protein-protein interactions with a kinesin light chain. In addition, GAR sequences mediate plasma membrane interactions of nucleolin. Both these modalities are in addition to the already reported involvement of the GAR domain in liquid-liquid phase separation in the nucleolus. Nucleolin transport to axons requires the GAR domain, and heterozygous GAR deletion mice reveal reduced axonal localization of nucleolin cargo mRNAs and enhanced sensory neuron growth. Thus, the GAR domain governs axonal transport of a growth controlling RNA-RBP complex in neurons, and is a versatile localization determinant for different subcellular compartments. Localization determination by GAR domains may explain why GAR mutants in diverse RBPs are associated with neurodegenerative disease.
Permanent Link: http://hdl.handle.net/11104/0322517
Research data: GitHub, ProteomeXchange, NCBI
Number of the records: 1