The proportion of the key components analysed in collagen-based isolates from fish and mammalian tissues processed by different protocols

0545736 - ÚSMH 2022 RIV US eng J - Journal Article
Rýglová, Šárka - Braun, Martin - Hříbal, Miloň - Suchý, Tomáš - Vöröš, Dominik
The proportion of the key components analysed in collagen-based isolates from fish and mammalian tissues processed by different protocols.
Journal of Food Composition and Analysis. Roč. 103, October (2021), č. článku 104059. ISSN 0889-1575. E-ISSN 1096-0481
R&D Projects: GA MZd(CZ) NV19-02-00068
Institutional support: RVO:67985891
Keywords : Collagen * Processing * Composition * Characterisation * Hydroxyproline * Lipids * Glycosaminoglycans
OECD category: Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
Impact factor: 4.520, year: 2021
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0889157521002593?via%3Dihub

The aim of this pilot study is to compare two extraction processes tested in our lab with intention to preserve the biological structure and functions of collagen-based isolate for biomedical purposes during its processing. Collagen type I was isolated from various animal sources from fish, porcine and rat skins. The resulting isolates, along with commercial calf collagens as control, were subsequently characterised and their composition examined by means of several analytical methods such as amino acid analysis, FTIR, SDS-PAGE. Moreover, the moisture, lipid, glycosaminoglycans, hydroxyproline and N contents were determined. We found that both the factors considered, the source of the collagen and the process employed, exerted effects on the final composition of the isolate and we monitored the key differences, in particular, with respect to the protein and lipid contents. Moreover, the study provides a discussion of issues surrounding the assessment of the actual pure collagen content determined on the basis of the content of hydroxyproline and the purity of the protein fraction.
Permanent Link: http://hdl.handle.net/11104/0322415