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A biorefinery approach to obtain docosahexaenoic acid and docosapentaenoic acid n-6 from Schizochytrium using high performance countercurrent chromatography
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SYSNO ASEP 0542764 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A biorefinery approach to obtain docosahexaenoic acid and docosapentaenoic acid n-6 from Schizochytrium using high performance countercurrent chromatography Author(s) Bárcenas-Pérez, Daniela (MBU-M) ORCID, RID
Lukeš, Martin (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCID
Kubáč, David (MBU-M) RID, ORCID
Kopecký, Jiří (MBU-M) ORCID
Kaštánek, P. (CZ)
Cheel, José (MBU-M) RID, ORCIDArticle number 102241 Source Title Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 55, MAY 2021 (2021)Number of pages 12 s. Language eng - English Country NL - Netherlands Keywords Docosahexaenoic acid (DHA) ; Docosapentaenoic acid n-6 (DPA n-6) ; Schizochytrium limacinum ; Countercurrent chromatography (CCC) ; High performance countercurrent ; chromatography (HPCCC) ; Centrifugal partition chromatography (CPC) Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000642452900004 EID SCOPUS 85101814625 DOI 10.1016/j.algal.2021.102241 Annotation Schizochytrium has arisen as a valuable alternative to fish as a source of polyunsaturated fatty acids. In the present study, Schizochytrium limacinum CO3-H biomass was processed by direct transesterification affording a transesterified algal oil, from which docosahexaenoic acid (DHA) and docosapentaenoic acid n-6 (DPA n-6) ethyl esters were isolated using high performance countercurrent chromatography (HPCCC). A multiple sequential injection HPCCC separation method was developed combining two elution modes (reverse phase and extrusion). During the reverse phase elution, the lower phase of a biphasic solvent system (n-heptane:acetonitrile, ratio 5:5, v/v) was used as the mobile phase. During the initial reverse phase elution, the two target compounds were obtained, which was followed by the extrusion of the stationary phase by switching the pumping of the mobile phase to the stationary phase, while maintaining the column rotation. Once the column was refilled with stationary phase, a new hydrodynamic equilibrium condition was again reached for a new separation cycle. Ten consecutive sample injections (1000 mg of algal oil, each) were performed in this way leading to the separation of DHA ethyl ester (1, 797 mg, 99% purity) and DPA n-6 ethyl ester (2, 164 mg, 97% purity) with recoveries of 99% and 92%, respectively. The process throughput of algal oil was 1.149 g/h while the efficiency per gram of two target compounds was 0.110 g/h. Environmental risk and process evaluation factors were used for evaluation of the separation process. Overall, this separation strategy may represent a useful model for the coproduction of DHA and DPA n-6 from microalgae oil under a biorefinery perspective because the potential coproduction of other valuable compounds present in both the algal oil and biomass may contribute to enhancement of the economics of the approach. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S2211926421000606?via%3Dihub#!
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