Number of the records: 1
Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies
- 1.
SYSNO ASEP 0537498 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies Author(s) Helmova, R. (CZ)
Hönig, Václav (BC-A) RID, ORCID
Tykalová, Hana (BC-A) RID, ORCID
Palus, Martin (BC-A) RID, ORCID
Bell-Sakyi, L. (GB)
Grubhoffer, Libor (BC-A) RID, ORCIDNumber of authors 6 Article number 902 Source Title Viruses. - : MDPI
Roč. 12, č. 8 (2020)Number of pages 17 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords nonstructural protein ns2a ; amino-acid substitution ; west-nile-virus ; swiss-model ; subcellular-localization ; 3'-untranslated region ; envelope glycoprotein ; noncoding region ; cell-lines ; dengue ; tbev ; host alternation ; neuroinvasiveness ; genome mutation ; quasispecies ; flavivirus adaptation ; tick cell line Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LTARF18021 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA20-30500S GA ČR - Czech Science Foundation (CSF) GA18-27204S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000579648500001 EID SCOPUS 85089798271 DOI 10.3390/v12080902 Annotation A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) andIxodes ricinustick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address https://www.mdpi.com/1999-4915/12/8/902
Number of the records: 1